Rabbit anti-Human OSBPL1A Polyclonal Antibody | anti-OSBPL1A antibody

OSBPL1A, CT (OSBPL1A, ORP1, OSBP8, OSBPL1, OSBPL1B, Oxysterol-binding protein-related protein 1) (Biotin)

Cat. Num. AAA6329704

• Gene Names: OSBPL1A; ORP1; ORP-1; OSBPL1B
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: OSBPL1A; Polyclonal Antibody; CT (OSBPL1A; ORP1; OSBP8; OSBPL1; OSBPL1B; Oxysterol-binding protein-related protein 1) (Biotin); Oxysterol-binding protein-related protein 1; anti-OSBPL1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 950

• Applicable Applications for anti-OSBPL1A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: OSBPL1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 734-763 amino acids from the C-terminal region of human OSBPL1A.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(OSBPL1A Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the OSBPL1A antibody detected the OSBPL1A protein (arrow).)

Related Product Information for anti-OSBPL1A antibody

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. Transcript variants derived from alternative promoter usage and/or alternative splicing exist; they encode different isoforms.

Product Categories/Family for anti-OSBPL1A antibody

Antibodies; Binding Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Hsu, Y.H., et al. PLoS Genet. 6 (6), E1000977 (2010): Rocha, N., et al. J. Cell Biol. 185(7):1209-1225(2009). Melzer, D., et al. PLoS Genet. 4 (5), E1000072 (2008): Bjornsson, H.T., et al. Genome Res. 18(5):771-779(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 19718741
• NCBI GeneID: 114876
• NCBI Accession #: NP_542164.2; NP_060500.3
• NCBI GenBank Nucleotide #: NP_542164.2
• UniProt Accession #: Q9BXW6
• NCBI Official Full Name: oxysterol-binding protein-related protein 1 isoform 2
• NCBI Official Synonym Full Names: oxysterol binding protein like 1A
• NCBI Official Symbol: OSBPL1A
• NCBI Official Synonym Symbols: ORP1; ORP-1; OSBPL1B
• NCBI Protein Information: oxysterol-binding protein-related protein 1
• UniProt Protein Name: Oxysterol-binding protein-related protein 1
• Protein Family: Oxysterol-binding protein-related protein
• UniProt Gene Name: OSBPL1A
• UniProt Synonym Gene Names: ORP1; OSBP8; OSBPL1; OSBPL1B; ORP-1; OSBP-related protein 1
• UniProt Entry Name: OSBL1_HUMAN

NCBI Description

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. Transcript variants derived from alternative promoter usage and/or alternative splicing exist; they encode different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

OSBPL1A: Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3- phosphate. Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A. Belongs to the OSBP family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 18q11.1

Cellular Component: nucleoplasm; lysosomal membrane; cytoplasm; late endosome; intracellular; nucleus

Molecular Function: protein binding; cholesterol binding; phospholipid binding

Biological Process: vesicle-mediated transport; cholesterol metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class II; lipid transport