Goat ORP3 Polyclonal Antibody | anti-OSBPL3 antibody

Goat anti-ORP3 Antibody

Cat. Num. AAA421424

• Gene Names: OSBPL3; ORP3; ORP-3; OSBP3
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: ORP3; Polyclonal Antibody; Goat anti-ORP3 Antibody; OSBPL3; ORP-3; OSBP3; KIAA0704; MGC21526; oxysterol binding protein-like 3; OSBP-related protein 3; oxysterol-binding protein 3; oxysterol-binding protein-related protein 3; DKFZp667P1518; OTTHUMP00000122490; OTTHUMP00000122491; OTTHUMP00000202019; OSBPL3 antibody; ORP3 antibody; ORP-3 antibody; OSBP3 antibody; KIAA0704 antibody; MGC21526 antibody; oxysterol binding protein-like 3 antibody; OSBP-related protein 3 antibody; oxysterol-binding protein 3 antibody; oxysterol-binding protein-related protein 3 antibody; DKFZp667P1518 antibody; OTTHUMP00000122490 antibody; OTTHUMP00000122491 antibody; OTTHUMP00000202019 antibody; oxysterol-binding protein-like protein 3 antibody; anti-OSBPL3 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: MSDEKNLGVSQKL
• Sequence Length: 887

• Applicable Applications for anti-OSBPL3 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: Preliminary experiments in Human Brain, Kidney, Muscle and Lung lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
• Immunogen: Peptide with sequence MSDEKNLGVSQKL-C, from the N Terminus of the protein sequence according to NP_056365.1; NP_663160.1; NP_663161.1; NP_663162.1.
• Epitope: N Terminus
• Note: This antibody is expected to recognise all reported isoforms (NP_056365.1; NP_663160.1; NP_663161.1; NP_663162.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 14149704
• NCBI GeneID: 26031
• NCBI Accession #: NP_056365.1; NP_663160.1; NP_663161.1; NP_663162.1
• NCBI GenBank Nucleotide #: NM_015550.3
• Molecular Weight: 64,776 Da
• NCBI Official Full Name: oxysterol-binding protein-related protein 3 isoform a
• NCBI Official Synonym Full Names: oxysterol binding protein like 3
• NCBI Official Symbol: OSBPL3
• NCBI Official Synonym Symbols: ORP3; ORP-3; OSBP3
• NCBI Protein Information: oxysterol-binding protein-related protein 3
• UniProt Protein Name: Oxysterol-binding protein-related protein 3
• Protein Family: Oxysterol-binding protein-related protein
• UniProt Gene Name: OSBPL3
• UniProt Synonym Gene Names: KIAA0704; ORP3; OSBP3; ORP-3; OSBP-related protein 3
• UniProt Entry Name: OSBL3_HUMAN

NCBI Description

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. The encoded protein is involved in the regulation of cell adhesion and organization of the actin cytoskeleton. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

Phosphoinositide-binding protein which associates with both cell and endoplasmic reticulum (ER) membranes (PubMed:16143324). Can bind to the ER membrane protein VAPA and recruit VAPA to plasma membrane sites, thus linking these intracellular compartments (PubMed:25447204). The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface (PubMed:18270267, PubMed:25447204). With VAPA, may regulate ER morphology (PubMed:16143324). Has a role in regulation of the actin cytoskeleton, cell polarity and cell adhesion (PubMed:18270267). Binds to phosphoinositides with preference for PI(3,4)P2 and PI(3,4,5)P3 (PubMed:16143324). Also binds 25-hydroxycholesterol and cholesterol (PubMed:17428193).