Western Blot (WB)
((0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Orexin receptor 1 Polyclonal Antibody | anti-HCRTR1 antibody
Goat anti-Orexin receptor 1 Antibody
Western Blot: Approx 48+35kDa band observed in Human Brain lysates (calculated MW of 47.5kDa according to NP_001516.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Silveyra et al, Am J Physiol Endocrinol Metab.2007 Oct;293(4): E977-85; PMID: 17638707). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B. [provided by RefSeq, Jan 2009]
Uniprot Description
HCRTR1: Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Seems to be exclusively coupled to the G(q) subclass of heteromeric G proteins, which activates the phospholipase C mediated signaling cascade. Belongs to the G-protein coupled receptor 1 family.
Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 1p33
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity; neuropeptide receptor activity; orexin receptor activity; peptide binding; peptide hormone binding
Biological Process: cellular response to hormone stimulus; feeding behavior; neuropeptide signaling pathway; regulation of circadian sleep/wake cycle, sleep; synaptic transmission
Research Articles on HCRTR1
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Product Notes
The HCRTR1 hcrtr1 (Catalog #AAA422563) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Orexin receptor 1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's Orexin receptor 1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 48+35kDa band observed in Human Brain lysates (calculated MW of 47.5kDa according to NP_001516.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Silveyra et al, Am J Physiol Endocrinol Metab.2007 Oct;293(4): E977-85; PMID: 17638707). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the HCRTR1 hcrtr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RNWKRPSDQL GD. It is sometimes possible for the material contained within the vial of "Orexin receptor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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