Mouse anti-Human ORC6L Monoclonal Antibody | anti-ORC6L antibody

ORC6L (Origin Recognition Complex Subunit 6-like, Origin Recognition Complex Subunit 6 Homolog-like (yeast), Origin Recognition Complex Subunit 6 Homolog-like 2, Origin Recognition Complex Subunit 6, ORC6) APC

Cat. Num. AAA6138019

• Gene Names: ORC6; ORC6L
• Reactivity: Human
• Applications: ELISA, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ORC6L; Monoclonal Antibody; ORC6L (Origin Recognition Complex Subunit 6-like; Origin Recognition Complex Subunit 6 Homolog-like (yeast); Origin Recognition Complex Subunit 6 Homolog-like 2; Origin Recognition Complex Subunit 6; ORC6) APC; anti-ORC6L antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG3,k
• Clone Number: 3C4
• Specificity: Recognizes human ORC6L.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 1613

• Applicable Applications for anti-ORC6L antibody: ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full-length recombinant protein corresponding to aa1-253 from ORC6L (AAH63565) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MGSELIGRLAPRLGLAEPDMLRKAEEYLRLSRVKCVGLSARTTETSSAVMCLDLAASWMKCPLDRAYLIKLSGLNKETYQSCLKSFECLLGLNSNIGIRDLAVQFSCIEAVNMASKILKSYESSLPQTQQVDLDLSRPLFTSAALLSACKILKLKVDKNKMVATSGVKKAIFDRLCKQLEKIGQQVDREPGDVATPPRKRKKIVVEAPAKEMEKVEEMPHKPQKDEDLTQDYEEWKRKILENAASAQKATAE*
• Conjugate: APC
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (53.83kD).)

Western Blot (WB)

(Western Blot analysis of ORC6L expression in transfected 293T cell line by ORC6L monoclonal antibody. Lane 1: ORC6L transfected lysate (28.1kD). Lane 2: Non-transfected lysate.)

Immunoprecipitation (IP)

(Immunoprecipitation of ORC6L transfected lysate using ORC6L monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ORC6L rabbit polyclonal antibody)

Related Product Information for anti-ORC6L antibody

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. It has been shown that this protein and and ORC1L are loosely associated with the core complex consisting of ORC2L, -3L, -4L and -5L. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis.

Product Categories/Family for anti-ORC6L antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 39795362
• NCBI GeneID: 23594
• NCBI Official Full Name: Homo sapiens origin recognition complex, subunit 6 like (yeast), mRNA
• NCBI Official Synonym Full Names: origin recognition complex subunit 6
• NCBI Official Symbol: ORC6
• NCBI Official Synonym Symbols: ORC6L
• NCBI Protein Information: origin recognition complex subunit 6

NCBI Description

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis. [provided by RefSeq, Oct 2010]