Rabbit anti-Mouse ORC5L Polyclonal Antibody | anti-ORC5L antibody

ORC5L, ID (ORC5, ORC5L, Origin recognition complex subunit 5) (MaxLight 405)

Cat. Num. AAA6329630

• Gene Names: ORC5; ORC5L; ORC5P; ORC5T; PPP1R117
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ORC5L; Polyclonal Antibody; ID (ORC5; Origin recognition complex subunit 5) (MaxLight 405); ORC5; Origin recognition complex subunit 5; anti-ORC5L antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes ORC5L. Species crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-ORC5L antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 227-256 in the central region of human ORC5L. Species sequence: mouse 100%
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis in mouse brain tissue lysates (35ug/lane) usingMBS6005580. This demonstrates MBS6005580 detected the ORC5L protein (arrow).)

Related Product Information for anti-ORC5L antibody

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq].

Product Categories/Family for anti-ORC5L antibody

Antibodies; Proteins

References

1. Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). 2. Trynka, G., et al. Gut 58(8):1078-1083(2009). 3. Siddiqui, K., et al. J. Biol. Chem. 282(44):32370-32383(2007). 4. Clarke, C.A., et al. Biochem. J. 388 (PT 2), 705-712 (2005). 5. Volkening, M., et al. Mol. Cell. Biol. 25(4):1560-1568(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4505525
• NCBI GeneID: 5001
• NCBI Accession #: NP_002544.1
• NCBI GenBank Nucleotide #: NM_002553.3
• UniProt Accession #: O43913; O60590; O95268; A4D0P8
• Molecular Weight: 37,414 Da
• NCBI Official Full Name: origin recognition complex subunit 5 isoform 1
• NCBI Official Synonym Full Names: origin recognition complex, subunit 5
• NCBI Official Symbol: ORC5
• NCBI Official Synonym Symbols: ORC5L; ORC5P; ORC5T; PPP1R117
• NCBI Protein Information: origin recognition complex subunit 5; protein phosphatase 1, regulatory subunit 117
• UniProt Protein Name: Origin recognition complex subunit 5
• UniProt Gene Name: ORC5
• UniProt Synonym Gene Names: ORC5L
• UniProt Entry Name: ORC5_HUMAN

Uniprot Description

ORC5L: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Belongs to the ORC5 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: nucleoplasm; cytoplasm; nuclear origin of replication recognition complex; nucleus; origin recognition complex

Molecular Function: protein binding; nucleotide binding; DNA replication origin binding; ATP binding

Biological Process: DNA replication initiation; mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle