Rabbit anti-Human, Mouse OPRM1 Polyclonal Antibody | anti-OPRM1 antibody

OPRM1, ID (OPRM1, MOR1, Mu-type opioid receptor, Mu opiate receptor, Mu opioid receptor) (APC)

Cat. Num. AAA6327866

• Gene Names: OPRM1; MOP; MOR; LMOR; MOR1; OPRM; M-OR-1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: OPRM1; Polyclonal Antibody; ID (OPRM1; MOR1; Mu-type opioid receptor; Mu opiate receptor; Mu opioid receptor) (APC); Mu opioid receptor; anti-OPRM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human OPRM1. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-OPRM1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 161-187 in the central region of human OPRM1. Species sequence homology: monkey, porcine and rat
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of mouse heart tissue lysate (35ug/lane) usingMBS6012405.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin fixed and paraffin embedded human brain tissue usingMBS6012405. Peroxidase-conjugated secondary antibody and DAB staining were used for visualization of the antigen. )

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of K562 cells using MBS6012405 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibody was used for the analysis.)

Product Categories/Family for anti-OPRM1 antibody

Antibodies; Opioid Receptors; Agonists

References

1. Bare, L.A., et.al., FEBS Lett. 354 (2), 213-216 (1994). 2. Wang, J.B., et.al., FEBS Lett. 338 (2), 217-222 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 117940060
• NCBI GeneID: 4988
• NCBI Accession #: NP_000905.3
• NCBI GenBank Nucleotide #: NM_000914.3
• UniProt Accession #: P35372; Q12930; Q4VWM1; Q4VWM2; Q4VWM3; B0FXJ1; B2R9S7; B8Q1L7; B8Q1L8; B8Q1L9; G8XRH6; G8XRH8
• Molecular Weight: 44,779 Da
• NCBI Official Full Name: mu-type opioid receptor isoform MOR-1
• NCBI Official Synonym Full Names: opioid receptor, mu 1
• NCBI Official Symbol: OPRM1
• NCBI Official Synonym Symbols: MOP; MOR; LMOR; MOR1; OPRM; M-OR-1
• NCBI Protein Information: mu-type opioid receptor; mu opiate receptor; mu opioid receptor hMOR-1a
• UniProt Protein Name: Mu-type opioid receptor
• Protein Family: Mu-type opioid receptor
• UniProt Gene Name: OPRM1
• UniProt Synonym Gene Names: MOR1; M-OR-1; MOR-1; MOP; hMOP
• UniProt Entry Name: OPRM_HUMAN

NCBI Description

This gene encodes one of three opioid receptors. The mu opioid receptor is the principal target of endogenous opioid peptides and opioid analgesic agents such a s beta-endorphn and enkephalins. The NM_001008503.1:c.118A>G allele had been associated with opioid and alcohol addiction and variations in pain sensitivity but evidence is conflicting. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

Uniprot Description

MOR-1: a Gi-protein-coupled receptor for beta-endorphin, morphine and other opiates. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Ligand-binding inactivates adenylyl cyclase, and activates a variety of G-beta-gamma-dependent pathways including the MAPK and the PI3K/Akt cascades. Two splice-variant isoforms have been described.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 6q24-q25

Cellular Component: dendrite cytoplasm; Golgi apparatus; neuron projection; focal adhesion; endoplasmic reticulum; integral to plasma membrane; plasma membrane; perikaryon; sarcolemma; lipid raft

Molecular Function: voltage-gated calcium channel activity; G-protein coupled receptor activity; protein domain specific binding; neuropeptide binding; protein binding; G-protein beta-subunit binding; G-protein alpha-subunit binding; beta-endorphin receptor activity; filamin binding

Biological Process: positive regulation of nitric oxide biosynthetic process; wound healing; cellular response to stress; locomotory behavior; response to lipopolysaccharide; behavioral response to ethanol; sensory perception of pain; synaptic transmission; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; neuropeptide signaling pathway; sensory perception; positive regulation of appetite; dopamine receptor, adenylate cyclase activating pathway; acute inflammatory response to antigenic stimulus; response to food; positive regulation of neurogenesis; negative regulation of nitric oxide biosynthetic process; negative regulation of adenylate cyclase activity; response to cocaine; G-protein signaling, coupled to cyclic nucleotide second messenger; reduction of cytosolic calcium ion concentration; opioid receptor, adenylate cyclase inhibiting pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential

Disease: Epilepsy, Idiopathic Generalized