Rabbit anti-Human NAB2 Polyclonal Antibody | anti-NAB2 antibody

Anti-NAB2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103931

• Gene Names: NAB2; MADER
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: NAB2; Polyclonal Antibody; Anti-NAB2 Antibody; Rabbit Polyclonal; NAB2 Antibody; Rabbit PAb; Antigen Affinity Purified; NGFI-A binding protein 2 (EGR1 binding protein 2); Anti-MADER Antibody; anti-NAB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NAB2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-NAB2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Human NAB2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human NAB2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NAB2 rabbit polyclonal antibody at 1:500 dilution Lane A: HT29 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:57 kDa Observed band size:57 kDa)

Related Product Information for anti-NAB2 antibody

Nab2 is constructed from an N-terminal PWI-fold domain, followed by QQQP and RGG motifs and then seven CCCH Zn fingers. The nuclear pore-associated proteins Gfd1 and Mlp1 bind to opposite sides of the Nab2 N-terminal domain and function in the nuclear export of mRNA, whereas the Zn fingers bind to A-rich regions of mature transcripts and function to regulate poly(A) tail length as well as mRNA compaction before nuclear export. NGF1-A-binding protein 2 (Nab2) is a conserved transcriptional cofactor that directly binds to Egr-1 and positively or negatively modulates Egr-1 target gene transcription. Nab2 is a novel endogenous negative regulator of Egr-1-dependent TGF-beta signaling responsible for setting the intensity of fibrotic responses. Defective Nab2 expression or function in dermal fibroblasts might play a role in persistent fibrotic responses in scleroderma. Mutations in the gene encoding the human Nab2 orthologue ZC3H14 and cause intellectual disability.

References

Oberson A, et al. (2018) Nab2 is a novel immune stimulator of mda-5 that promotes a strong type i interferon response. Oncotarget 9 (5): 5641-5651.Fasken MB, et al. (2019) Structure-function relationships in the nab2 polyadenosine-rna binding zn finger protein family. Protein Sci 28 (3): 513-523.Bhattacharyya S, et al. (2009) The transcriptional cofactor nab2 is induced by tgf-beta and suppresses fibroblast activation: Physiological roles and impaired expression in scleroderma. PLoS One 4 (10): e7620.

NCBI and Uniprot Product Information

• NCBI GI #: 5174607
• NCBI GeneID: 4665
• NCBI Accession #: NP_005958.1
• NCBI GenBank Nucleotide #: NM_005967.3
• UniProt Accession #: Q15742; O76006; Q14797; B2RAK3
• Molecular Weight: 56,594 Da
• NCBI Official Full Name: NGFI-A-binding protein 2
• NCBI Official Synonym Full Names: NGFI-A binding protein 2 (EGR1 binding protein 2)
• NCBI Official Symbol: NAB2
• NCBI Official Synonym Symbols: MADER
• NCBI Protein Information: NGFI-A-binding protein 2; EGR1 binding protein 2; EGR-1-binding protein 2; melanoma-associated delayed early response protein
• UniProt Protein Name: NGFI-A-binding protein 2
• Protein Family: NGFI-A-binding protein
• UniProt Gene Name: NAB2
• UniProt Synonym Gene Names: MADER; Protein MADER
• UniProt Entry Name: NAB2_HUMAN

NCBI Description

This gene encodes a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by the encoded protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

NAB2: Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability. Belongs to the NAB family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: nucleus

Molecular Function: transcription factor binding; transcription corepressor activity

Biological Process: Schwann cell differentiation; myelination; cell proliferation; nervous system development; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase III promoter; regulation of epidermis development; endochondral ossification