Rabbit anti-Human OPN-a/b Polyclonal Antibody | anti-OPN-a/b antibody

OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (MaxLight 650)

Cat. Num. AAA6327840

• Gene Names: SPP1; OPN; BNSP; BSPI; ETA-1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: OPN-a/b; Polyclonal Antibody; NT (SPP1; BNSP; OPN; Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; Urinary stone protein; Uropontin) (MaxLight 650); SPP1; Uropontin; anti-OPN-a/b antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human OPN-a/b.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-OPN-a/b antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 14-40 in the N-terminal region of human OPN-a/b, UniProt Accession #P10451.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of OPN-a/b (arrow) usingMBS648553. 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the OPN-a/b gene.)

Testing Data

(All lanes: MBS648553 at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: MOLT-4 whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG (H+L) goat anti-rabbit, Peroxidase conjugated at 1:10000 dilution. Predicted band size: 35kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data

(039288 at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG, (H+L) goat anti-rabbit Peroxidase conjugated at 1:10000 dilution. Predicted band size: 35kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data

(All lanes: MBS648553 at 1:1000 dilution Lane 1: human liver lysates Lane 2: MDA-MB-453 whole cell lysates Lane 3: MOLT-4 whole cell lysates Lysates/proteins at 20ug/lane. Secondary IgG, (H+L) goat anti-rabbit, Peroxidase conjugated at 1:10000 dilution. Predicted band size : 35kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS648553 followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBS648553 for immunohistochemistry.)

Immunofluorescence (IF)

(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with MBS648553 at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)

Related Product Information for anti-OPN-a/b antibody

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity).

Product Categories/Family for anti-OPN-a/b antibody

Antibodies; Osteopontin

References

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Weber, G.F., et al. Br. J. Cancer 103(6):861-869(2010). 3. Chen, R.X., et al. J. Gastroenterol. Hepatol. 25(8):1435-1442(2010). 4. Jugessur, A., et al. PLoS ONE 5(7), E11493 (2010). 5. Johnatty, S.E., et al. PLoS Genet. 6(7), E1001016 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4759166
• NCBI GeneID: 6696
• NCBI Accession #: NP_000573.1; NP_001035147.1
• NCBI GenBank Nucleotide #: NM_000582.2
• UniProt Accession #: P10451; Q15681; Q15682; Q15683; Q4W597; Q567T5; Q8NBK2; Q96IZ1; B2RDA1
• Molecular Weight: 35,423 Da
• NCBI Official Full Name: osteopontin isoform OPN-b
• NCBI Official Synonym Full Names: secreted phosphoprotein 1
• NCBI Official Symbol: SPP1
• NCBI Official Synonym Symbols: OPN; BNSP; BSPI; ETA-1
• NCBI Protein Information: osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I,
• UniProt Protein Name: Osteopontin
• UniProt Gene Name: SPP1
• UniProt Synonym Gene Names: BNSP; OPN; SPP-1
• UniProt Entry Name: OSTP_HUMAN

NCBI Description

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: extracellular space; cell projection; perinuclear region of cytoplasm; apical part of cell; extracellular region

Molecular Function: protein binding; extracellular matrix binding; cytokine activity

Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; decidualization; osteoblast differentiation; extracellular matrix disassembly; response to vitamin D; biomineral formation; response to steroid hormone stimulus; negative regulation of collateral sprouting of intact axon in response to injury; positive regulation of bone resorption; inflammatory response; cell adhesion; embryo implantation