Rabbit Opioid Receptor Delta Polyclonal Antibody | anti-OPRD1 antibody
Rabbit Anti-Mu Opioid Receptor Serum
Gene Names
OPRD1; OPRD
Applications
Immunoblot, Western Blot, Western Blot
Synonyms
Opioid Receptor Delta; Polyclonal Antibody; Rabbit Anti-Mu Opioid Receptor Serum; Opioid Mu Receptor (391-398); anti-OPRD1 antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Polypeptide ::: % Cross Reactivity
Mu Opioid Receptor (391-398) ::: 100
Mu Opioid Receptor ::: 50
Mu Opioid Receptor (391-398) ::: 100
Mu Opioid Receptor ::: 50
Form/Format
Antiserum Specificity
Sequence Length
372
Applicable Applications for anti-OPRD1 antibody
Immunocytochemical Staining, Western Immunoblot, Western Blot
Application Notes
Immunocytochemistry: This antiserum has been found to stain specific cells in various regions of PLP fixed rat brain sections known to express the mu opioid receptor using ABC techniques at a dilution of 1:2000..
Western Blot: Western immunoblots using whole rat brain homogenate have been successful at a dilution of 1:500.
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the rabbit polyclonal antibody after dilution to at least 1:500 (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 4 for 5 minute/wash with TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.
Western Blot: Western immunoblots using whole rat brain homogenate have been successful at a dilution of 1:500.
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the rabbit polyclonal antibody after dilution to at least 1:500 (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 4 for 5 minute/wash with TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.
Related Product Information for anti-OPRD1 antibody
The antiserum was raised in a rabbit which was immunized with a peptide analogue of the carboxyl terminal of the mu opioid receptor covalently attached onto a carrier protein. This antiserum is specific for the COOH terminal of the mu opioid receptor and is suitable for immunocytochemical and western immunoblotting detection of the receptor. The antiserum has been characterized by western blotting. ELISA. and cell staining techniques. Dilute the lyophilized antiserum with 0.1 ml of 10 mg/ml BSA is PBS for the equivalent of whole antiserum. or with additional buffer for more dilute antiserum.
Product Categories/Family for anti-OPRD1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40238 Da
NCBI Official Full Name
delta-type opioid receptor
NCBI Official Synonym Full Names
opioid receptor, delta 1
NCBI Official Symbol
OPRD1
NCBI Official Synonym Symbols
OPRD
NCBI Protein Information
delta-type opioid receptor; DOR-1; D-OR-1; OTTHUMP00000003778; delta opioid receptor 1
UniProt Protein Name
Delta-type opioid receptor
Protein Family
UniProt Gene Name
OPRD1
UniProt Synonym Gene Names
OPRD
UniProt Entry Name
OPRD_HUMAN
Uniprot Description
Function: Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Highly stereoselective. receptor for enkephalins.
Subunit structure: Interacts with GPRASP1. Ref.6 Ref.7
Subcellular location: Cell membrane; Multi-pass membrane protein.
Sequence similarities: Belongs to the G-protein coupled receptor 1 family.
Research Articles on OPRD1
Similar Products
Product Notes
The OPRD1 oprd1 (Catalog #AAA352197) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Opioid Receptor Delta can be used in a range of immunoassay formats including, but not limited to, Immunocytochemical Staining, Western Immunoblot, Western Blot. Immunocytochemistry: This antiserum has been found to stain specific cells in various regions of PLP fixed rat brain sections known to express the mu opioid receptor using ABC techniques at a dilution of 1:2000. Western Blot: Western immunoblots using whole rat brain homogenate have been successful at a dilution of 1:500. Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer. 2. Wash x 2 with TBS/Tween-20. 3. Apply the rabbit polyclonal antibody after dilution to at least 1:500 (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours. 4. Wash x 3 with TBS/Tween-20. 5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques. 6. Wash x 4 for 5 minute/wash with TBS/Tween-20. 7. Develop color using the enhanced DAB reaction. Researchers should empirically determine the suitability of the OPRD1 oprd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Opioid Receptor Delta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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