Rabbit Opioid Receptor 1, mu, aa384-398 Polyclonal Antibody | anti-OPRM1 antibody
Opioid Receptor 1, mu, aa384-398 (MOR1)
Serum
Serum
Dilution: Immunohistochemistry: 1:500-1:1000 (Cy3 technique); 1:6000-1:10,000 (Biotin/avidin-HRP technique).
Dilute antibody in PBS, 0.3% Triton X-100. Incubate 18-24 hrs at 2-8 degree C. Perfusion Fixation: With 500ml 0.1M phosphate buffer, pH 7.4, 4% paraformaldehyde for 20-30 minutes. Post Fixation: 0.1M phosphate buffer, pH 7.4, 4% paraformaldehyde for 1.5 hours at 4 degree C. Sections: 10um cryostate. Note that a change in the fixation or buffering system as used in our protocol may change the configuration of the protein, and therefore, may alter the reactivity with the tissue tested. Pre-adsorption with immunogen peptide at 10ug/ml completely eliminates labeling.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of three opioid receptors. The mu opioid receptor is the principal target of endogenous opioid peptides and opioid analgesic agents such a s beta-endorphn and enkephalins. The NM_001008503.1:c.118A>G allele had been associated with opioid and alcohol addiction and variations in pain sensitivity but evidence is conflicting. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
Uniprot Description
MOR-1: a Gi-protein-coupled receptor for beta-endorphin, morphine and other opiates. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Ligand-binding inactivates adenylyl cyclase, and activates a variety of G-beta-gamma-dependent pathways including the MAPK and the PI3K/Akt cascades. Two splice-variant isoforms have been described.
Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR
Chromosomal Location of Human Ortholog: 6q24-q25
Cellular Component: dendrite cytoplasm; Golgi apparatus; neuron projection; focal adhesion; integral to plasma membrane; endoplasmic reticulum; plasma membrane; perikaryon; sarcolemma; lipid raft
Molecular Function: G-protein coupled receptor activity; voltage-gated calcium channel activity; neuropeptide binding; protein domain specific binding; protein binding; G-protein alpha-subunit binding; G-protein beta-subunit binding; beta-endorphin receptor activity; filamin binding
Biological Process: positive regulation of nitric oxide biosynthetic process; wound healing; cellular response to stress; response to lipopolysaccharide; locomotory behavior; behavioral response to ethanol; sensory perception of pain; synaptic transmission; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; neuropeptide signaling pathway; sensory perception; positive regulation of appetite; acute inflammatory response to antigenic stimulus; dopamine receptor, adenylate cyclase activating pathway; response to food; positive regulation of neurogenesis; negative regulation of nitric oxide biosynthetic process; negative regulation of adenylate cyclase activity; response to cocaine; G-protein signaling, coupled to cyclic nucleotide second messenger; reduction of cytosolic calcium ion concentration; opioid receptor, adenylate cyclase inhibiting pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential
Disease: Epilepsy, Idiopathic Generalized
Research Articles on OPRM1
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Product Notes
The OPRM1 oprm1 (Catalog #AAA627618) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Opioid Receptor 1, mu, aa384-398 (MOR1) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Opioid Receptor 1, mu, aa384-398 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Immunohistochemistry, and Western Blot. Dilution: Immunohistochemistry: 1:500-1:1000 (Cy3 technique); 1:6000-1:10,000 (Biotividin-HRP technique). Dilute antibody in PBS, 0.3% Triton X-100. Incubate 18-24 hrs at 2-8 degree C. Perfusion Fixation: With 500ml 0.1M phosphate buffer, pH 7.4, 4% paraformaldehyde for 20-30 minutes. Post Fixation: 0.1M phosphate buffer, pH 7.4, 4% paraformaldehyde for 1.5 hours at 4 degree C. Sections: 10um cryostate. Note that a change in the fixation or buffering system as used in our protocol may change the configuration of the protein, and therefore, may alter the reactivity with the tissue tested. Pre-adsorption with immunogen peptide at 10ug/ml completely eliminates labeling. Researchers should empirically determine the suitability of the OPRM1 oprm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Opioid Receptor 1, mu, aa384-398, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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