Human IL-4 ELISA Kit | IL-4 elisa kit

Human IL-4 ELISA KIT

Cat. Num. AAA9424796

• Gene Names: IL4; IL-4
• Reactivity: Human
• Synonyms: IL-4; Human IL-4 ELISA KIT; Human IL-4; IL-4 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human IL-4 Ligand

• Assay Type: Solid Phase Sandwich ELISA
• Samples: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
• Detection Range: 7.8-500pg/mL
• Sensitivity: 2pg/mL
• Assay Time: 3 hours
• Sample Volume: 20 uL
• Detection Method: Colorimetric
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

(Representative standard curve for IL-4 ELISA. IL-4 was diluted in serial two-fold steps in Sample Diluent.)

Related Product Information for IL-4 elisa kit

Interleukin 4 (IL-4) is a pleiotropic cytokine produced primarily by activated T lymphocytes, mast cells and basophils (1-3). The sequence of human IL-4 cDNA predicts a 153 amino acid (aa) residue precursor protein containing a 24 aa residue signal peptide that is cleaved to form the mature protein (4). At the amino acid sequence level, mature human IL-4 is approximately 50% identical to mouse IL-4 and there is no species cross-reactivity between the two proteins (1, 2). Human IL-4 also shares approximately 30% amino acid sequence identity to human IL-13 and the two cytokines exhibit overlapping biological activities (5, 6). The gene for IL-4 has been mapped to human chromosome 5q, in close proximity to the genes for IL-3, IL-5, IL-13 and GM-CSF (1, 2).IL-4 has multiple immune response-modulating functions on a variety of cell types. It is an important regulator of isotype switching, inducing IgE production in B lymphocytes. It is an important modulator of the differentiation of precursor T helper cells to the TH2 subset that mediates humoral immunity and modulates antibody production. In addition, IL-4 has also been shown to have anti-tumor activity both in vivo and in vitro (1-3).The biological effects of IL-4 are mediated by specific cell surface receptor complexes. One type of functional IL-4 receptor complex consists of the IL-4-binding subunit (IL-4 R) and a second chain, designated the common c chain because it has also been identified as a component of the receptor complexes for IL-2, IL-7, IL-9 and IL-15 (7-9). A second type of functional IL-4 receptor complex, consisting of the IL-4 R and the more recently cloned IL-13 R, has also been proposed (10, 11).Although IL-4 R does not bind IL-13 directly, it has been shown to complex with the low-affinity IL-13 R to form the functional high-affinity receptor complex for IL-13 (11, 12). In addition to the membrane-bound form of IL-4 R, a naturally occurring soluble form of IL-4 R has been identified in human and mouse biological fluids and in mouse cell culture supernates (13-15). Soluble IL-4 R has been to shown to bind IL-4 with high affinity in solution.

NCBI and Uniprot Product Information

• NCBI GI #: 21264449
• NCBI GeneID: 443327
• UniProt Accession #: P30368; Q95MZ6
• Molecular Weight: 15,136 Da
• NCBI Official Full Name: Interleukin-4
• NCBI Official Symbol: IL4
• NCBI Official Synonym Symbols: IL-4
• NCBI Protein Information: interleukin-4
• UniProt Protein Name: Interleukin-4
• UniProt Gene Name: IL4
• UniProt Synonym Gene Names: IL-4; IL-4; BSF-1

Uniprot Description

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages.