Chicken Methylase ELISA Kit | Methylase elisa kit
Chicken Methylase ELISA Kit
Reactivity
Chicken
Synonyms
Methylase; Chicken Methylase ELISA Kit; Methylase elisa kit
Reactivity
Chicken
Specificity
This kit recognizes natural and recombinant Chicken Methylase. No significant cross-reactivity or interference between Chicken Methylase and analogues was observed.
Sequence Length
428
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
78.125-5000pg/mL
Sensitivity
Min: 46.875pg/mL; Max: 5000pg/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Methylase elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken Methylase concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Methylase. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Methylase and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Methylase, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Methylase. You can calculate the concentration of Methylase in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Methylase. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Methylase and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Methylase, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Methylase. You can calculate the concentration of Methylase in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
48,408 Da
NCBI Official Full Name
methylase
UniProt Protein Name
Modification methylase TthHB8I
UniProt Gene Name
tthHB8IM
UniProt Synonym Gene Names
M.TthHB8I
UniProt Entry Name
MTT8_THET8
Uniprot Description
This methylase recognizes the double-stranded sequence TCGA, causes specific methylation on A-4 on both strands and protects the DNA from cleavage by the TthHB8I endonuclease.
