Immunofluorescence (IF)
(Immunofluorescence staining of OLIG1 in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human OLIG1 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.)
Rabbit anti-Human Olig1 Polyclonal Antibody | anti-OLIG1 antibody
Anti-Olig1 Antibody, Rabbit Polyclonal
ICC/IF: 1:1500-1:50,000
IP: 0.2-1uL/mg of lysate
Immunofluorescence (IF)
(Immunofluorescence staining of OLIG1 in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human OLIG1 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of OLIG1 in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human OLIG1 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.)
Immunoprecipitation (IP)
(OLIG1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate0.5 uL anti-OLIG1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-OLIG1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa)
Immunoprecipitation (IP)
(OLIG1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate0.5 uL anti-OLIG1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-OLIG1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa)
Western Blot (WB)
(Anti-OLIG1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:35 kDa)
Western Blot (WB)
(Anti-OLIG1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:35 kDa)
NCBI and Uniprot Product Information
Observed: 29 kDa
Uniprot Description
OLIG1: Promotes formation and maturation of oligodendrocytes, especially within the brain. Cooperates with OLIG2 to establish the pMN domain of the embryonic neural tube.
Protein type: Transcription factor; Cell development/differentiation
Chromosomal Location of Human Ortholog: 21q22.11
Cellular Component: nucleus
Molecular Function: protein dimerization activity; protein binding; DNA binding
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; neuron fate commitment
Research Articles on OLIG1
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Product Notes
The OLIG1 olig1 (Catalog #AAA8100504) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Olig1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Olig1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:1000 ICC/IF: 1:1500-1:50,000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the OLIG1 olig1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Olig1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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