Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using OGFOD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)
Rabbit anti-Human OGFOD1 Polyclonal Antibody | anti-OGFOD1 antibody
OGFOD1 Rabbit pAb
Gene Names
OGFOD1; TPA1
Reactivity
Human
Applications
Western Blot
Purity
Affinity purification
Synonyms
OGFOD1; Polyclonal Antibody; OGFOD1 Rabbit pAb; TPA1; anti-OGFOD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence
MNGKRPAEPGPARVGKKGKKEVMAEFSDAVTEETLKKQVAEAWSRRTPFSHEVIVMDMDPFLHCVIPNFIQSQDFLEGLQKELMNLDFHEKYNDLYKFQQSDDLKKRREPHISTLRKILFEDFRSWLSDISKIDLESTID
Applicable Applications for anti-OGFOD1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human OGFOD1 (NP_060703.3).
Cellular Location
Cytoplasm, Nucleus
Positive Samples
HeLa
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using OGFOD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using OGFOD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)
Product Categories/Family for anti-OGFOD1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,861 Da
NCBI Official Full Name
prolyl 3-hydroxylase OGFOD1
NCBI Official Synonym Full Names
2-oxoglutarate and iron-dependent oxygenase domain containing 1
NCBI Official Symbol
OGFOD1
NCBI Official Synonym Symbols
TPA1
NCBI Protein Information
prolyl 3-hydroxylase OGFOD1; termination and polyadenylation 1 homolog; TPA1, termination and polyadenylation 1, homolog; 2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1
UniProt Protein Name
Prolyl 3-hydroxylase OGFOD1
Protein Family
UniProt Gene Name
OGFOD1
UniProt Synonym Gene Names
KIAA1612; TPA1
UniProt Entry Name
OGFD1_HUMAN
Uniprot Description
OGFOD1: Belongs to the TPA1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.11.-; Oxidoreductase
Chromosomal Location of Human Ortholog: 16q12.2
Cellular Component: stress granule; cytoplasm; nucleus
Molecular Function: peptidyl-proline dioxygenase activity; L-ascorbic acid binding; iron ion binding; peptidyl-proline 3-dioxygenase activity
Biological Process: cell proliferation; stress granule assembly; peptidyl-proline hydroxylation; protein amino acid hydroxylation; regulation of translational termination
Research Articles on OGFOD1
Similar Products
Product Notes
The OGFOD1 ogfod1 (Catalog #AAA9142355) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The OGFOD1 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OGFOD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the OGFOD1 ogfod1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MNGKRPAEPG PARVGKKGKK EVMAEFSDAV TEETLKKQVA EAWSRRTPFS HEVIVMDMDP FLHCVIPNFI QSQDFLEGLQ KELMNLDFHE KYNDLYKFQQ SDDLKKRREP HISTLRKILF EDFRSWLSDI SKIDLESTID. It is sometimes possible for the material contained within the vial of "OGFOD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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