Western Blot (WB)
(Western blot analysis of OGFOD1 in Daudi cell lysate with OGFOD1 antibody at 1 μg/mL.)
Rabbit anti-Human, Mouse OGFOD1 Polyclonal Antibody | anti-OGFOD1 antibody
OGFOD1 Antibody
Gene Names
OGFOD1; TPA1
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunofluorescence
Purity
OGFOD1 Antibody is affinity chromatography purified via peptide column.
Synonyms
OGFOD1; Polyclonal Antibody; OGFOD1 Antibody; TPA1; KIAA1612; 2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1; Termination and polyadenylation 1 homolog; 2-oxoglutarate and iron-dependent oxygenase domain containing 1; anti-OGFOD1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
OGFOD1 antibody is predicted to not cross-react with other TPA1 protein family members. At least two isoforms are known to exist; this antibody will only detect the larger isform.
Purity/Purification
OGFOD1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
542
Applicable Applications for anti-OGFOD1 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
OGFOD1 antibody can be used for detection of OGFOD1 by Western blot at 1 mug/mL. Antibody can also be used for immunofluorescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
OGFOD1 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human OGFOD1.
Buffer
OGFOD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
OGFOD1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of OGFOD1 in Daudi cell lysate with OGFOD1 antibody at 1 μg/mL.)
Western Blot (WB)
(Western blot analysis of OGFOD1 in Daudi cell lysate with OGFOD1 antibody at 1 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of OGFOD1 in Daudi cells with OGFOD1 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of OGFOD1 in Daudi cells with OGFOD1 antibody at 20 μg/mL.)
Related Product Information for anti-OGFOD1 antibody
OGFOD1 Antibody: OGFOD1, a member of the 2-OG-Fe(II) dioxygenase family, plays an important role in ischemic survival and stress. It is an oxidoreductase containing a Prolyl 4-hydroxylase alpha subunit. An OGFOD1 iron binding residue is required for ATPAF1 gene expression. As a novel stress granule component, OGFOD1 plays important proapoptotic roles in the regulation of translation and HRI-mediated phosphorylation of eIF2alpha in cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,861 Da
NCBI Official Full Name
prolyl 3-hydroxylase OGFOD1
NCBI Official Synonym Full Names
2-oxoglutarate and iron-dependent oxygenase domain containing 1
NCBI Official Symbol
OGFOD1
NCBI Official Synonym Symbols
TPA1
NCBI Protein Information
prolyl 3-hydroxylase OGFOD1; termination and polyadenylation 1 homolog; TPA1, termination and polyadenylation 1, homolog; 2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1
UniProt Protein Name
Prolyl 3-hydroxylase OGFOD1
Protein Family
UniProt Gene Name
OGFOD1
UniProt Synonym Gene Names
KIAA1612; TPA1
UniProt Entry Name
OGFD1_HUMAN
Uniprot Description
OGFOD1: Belongs to the TPA1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.11.-; Oxidoreductase
Chromosomal Location of Human Ortholog: 16q12.2
Cellular Component: stress granule; cytoplasm; nucleus
Molecular Function: peptidyl-proline dioxygenase activity; L-ascorbic acid binding; iron ion binding; peptidyl-proline 3-dioxygenase activity
Biological Process: cell proliferation; stress granule assembly; peptidyl-proline hydroxylation; protein amino acid hydroxylation; regulation of translational termination
Research Articles on OGFOD1
Similar Products
Product Notes
The OGFOD1 ogfod1 (Catalog #AAA151396) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The OGFOD1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's OGFOD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). OGFOD1 antibody can be used for detection of OGFOD1 by Western blot at 1 mug/mL. Antibody can also be used for immunofluorescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the OGFOD1 ogfod1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OGFOD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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