Western Blot (WB)
(Western BlotPositive WB detected in: NIH/3T3 whole cell lysateAll lanes: ODR4 antibody at 4.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 52, 49, 38, 48 KDaObserved band size: 52 KDa)
Rabbit anti-Human, Mouse ODR4 Polyclonal Antibody | anti-ODR4 antibody
ODR4 Antibody
Gene Names
ODR4; TTG1; odr-4; C1orf27
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
ODR4; Polyclonal Antibody; ODR4 Antibody; Protein odr-4 homolog; hODR-4; LAG1-interacting protein; Transactivated by transforming growth factor beta protein 1; C1orf27; TTG1; TTG1A; anti-ODR4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
431
Applicable Applications for anti-ODR4 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:5000
IHC: 1:200-1:500
IF: 1:50-1:200
IHC: 1:200-1:500
IF: 1:50-1:200
Immunogen
Recombinant Human Protein odr-4 homolog protein (153-251AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Western Blot (WB)
(Western BlotPositive WB detected in: NIH/3T3 whole cell lysateAll lanes: ODR4 antibody at 4.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 52, 49, 38, 48 KDaObserved band size: 52 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: NIH/3T3 whole cell lysateAll lanes: ODR4 antibody at 4.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 52, 49, 38, 48 KDaObserved band size: 52 KDa)
Immunohistochemistry (IHC)
(IHC image of MBS7109228 diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of MBS7109228 diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(Immunofluorescence staining of HepG2 cells with MBS7109228 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunohistochemistry (IHC)
(Immunofluorescence staining of HepG2 cells with MBS7109228 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Related Product Information for anti-ODR4 antibody
May play a role in the trafficking of a subset of G-protein coupled receptors.
Product Categories/Family for anti-ODR4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,593 Da
NCBI Official Full Name
protein odr-4 homolog isoform 2
NCBI Official Synonym Full Names
odr-4 GPCR localization factor homolog
NCBI Official Symbol
ODR4
NCBI Official Synonym Symbols
TTG1; odr-4; C1orf27
NCBI Protein Information
protein odr-4 homolog
UniProt Protein Name
Protein odr-4 homolog
Protein Family
UniProt Gene Name
ODR4
UniProt Synonym Gene Names
hODR-4
Uniprot Description
May play a role in the trafficking of a subset of G-protein coupled receptors.
Research Articles on ODR4
Similar Products
Product Notes
The ODR4 odr4 (Catalog #AAA7109228) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ODR4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ODR4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:5000 IHC: 1:200-1:500 IF: 1:50-1:200. Researchers should empirically determine the suitability of the ODR4 odr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ODR4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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