Rabbit Oct-1 Polyclonal Antibody | anti-Oct-1 antibody

Oct-1 (L68) Polyclonal Antibody

Cat. Num. AAA3000754

• Gene Names: POU2F1; OCT1; OTF1; oct-1B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Oct-1; Polyclonal Antibody; Oct-1 (L68) Polyclonal Antibody; POU domain; class 2; transcription factor 1; NF-A1; Octamer-binding protein 1; Octamer-binding transcription factor 1; OTF-1; POU2F1; OCT1; OTF1; anti-Oct-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: OCT (L68) polyclonal antibody detects endogenous levels of OCT1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 755

• Applicable Applications for anti-Oct-1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 30-80 of Human Oct-1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Oct-1 (L68) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:NIH-3T3 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of OCT1 (L68) pAb in paraffin-embedded human lymph node tissue.)

Related Product Information for anti-Oct-1 antibody

Organic cation transporters (OCT) are expressed in the plasma membrane of epithelial cells from a wide range of tissues, where they function in the elimination of endogenous amines, cationic drugs and other xenobiotics. In humans, OCT1 is primarily expressed in the liver while OCT2 is expressed in the kidney. OCT3 is expressed in the placenta, skeletal muscle, prostate, aorta and liver. OCT3, also known as extraneuronal monoamine transporter, is widely expressed in different regions of the brain including the hippocampus, cerebellum and cerebral cortex. OCT3 mediates the uptake of several neuroactive agents, including dopamine, and may play an important role in the disposition of neurotransmitters and cationic neurotoxins in the brain.

NCBI and Uniprot Product Information

• NCBI GI #: 311771667
• NCBI GeneID: 5451
• NCBI Accession #: NP_001185712.1
• NCBI GenBank Nucleotide #: NP_001185712.1
• UniProt Accession #: P14859
• Molecular Weight: ~ 89kDa
• NCBI Official Full Name: POU domain, class 2, transcription factor 1 isoform 2
• NCBI Official Synonym Full Names: POU class 2 homeobox 1
• NCBI Official Symbol: POU2F1
• NCBI Official Synonym Symbols: OCT1; OTF1; oct-1B
• NCBI Protein Information: POU domain, class 2, transcription factor 1
• UniProt Protein Name: POU domain, class 2, transcription factor 1
• UniProt Gene Name: POU2F1
• UniProt Synonym Gene Names: OCT1; OTF1; Oct-1; OTF-1
• UniProt Entry Name: PO2F1_HUMAN

NCBI Description

The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]

Uniprot Description

Oct1: a ubiquitous transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR. Phosphorylation apparently inhibits its interaction with DNA. Four splice variant isoforms have been described. Isoform 2 is lymphocyte specific.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: nucleoplasm; transcription factor complex; intracellular membrane-bound organelle; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase III promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; olfactory placode formation