Rabbit OBFC2B Polyclonal Antibody | anti-OBFC2B antibody

OBFC2B Antibody

Cat. Num. AAA9611952

• Gene Names: NABP2; SSB1; hSSB1; OBFC2B; SOSS-B1
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: OBFC2B; Polyclonal Antibody; OBFC2B Antibody; 2610036N15Rik; hSSB1; LP3587; MGC125161; MGC2731; MGC30353; Nabp2; Nucleic acid binding protein 2; Oligonucleotide/oligosaccharide binding fold containing 2B; Oligonucleotide/oligosaccharide binding fold containing protein 2B; RGD1308158; Sensor of single strand DNA complex subunit B1; Sensor of ssDNA subunit B1; Single stranded DNA binding protein 1; SOSS complex subunit B1; SSB1; anti-OBFC2B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: OBFC2B Antibody detects endogenous levels of OBFC2B.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 211

• Applicable Applications for anti-OBFC2B antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human OBFC2B.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using OBFC2B Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-OBFC2B antibody

Description: Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The SOSS complex associates with DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. Required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways.

Post Translational Modifications: Phosphorylated by ATM in response to DNA damage. Phosphorylation prevents degradation by the proteasome, hence stabilization of the protein and accumulation within cells.

Subunit Structure: Component of the SOSS complex, composed of SOSS-B (SOSS-B1/NABP2 or SOSS-B2/NABP1), SOSS-A/INTS3 and SOSS-C/INIP. SOSS complexes containing SOSS-B1/NABP2 are more abundant than complexes containing SOSS-B2/NABP1. Directly interacts with ATM, SOSS-A/INTS3 and RAD51. Interacts with INTS7.

Similarity: Belongs to the SOSS-B family. SOSS-B1 subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 13129048
• NCBI GeneID: 79035
• NCBI Accession #: NP_076973
• NCBI GenBank Nucleotide #: NP_076973.1
• UniProt Accession #: Q9BQ15
• Molecular Weight: Observed: 32 kDa; Predicted: 23 kDa
• NCBI Official Full Name: SOSS complex subunit B1
• NCBI Official Synonym Full Names: nucleic acid binding protein 2
• NCBI Official Symbol: NABP2
• NCBI Official Synonym Symbols: SSB1; hSSB1; OBFC2B; SOSS-B1
• NCBI Protein Information: SOSS complex subunit B1
• UniProt Protein Name: SOSS complex subunit B1
• UniProt Gene Name: NABP2
• UniProt Synonym Gene Names: OBFC2B; SSB1; SOSS-B1; hSSB1
• UniProt Entry Name: SOSB1_HUMAN

NCBI Description

Single-stranded DNA (ssDNA)-binding proteins, such as OBFC2B, are ubiquitous and essential for a variety of DNA metabolic processes, including replication, recombination, and detection and repair of damage (Richard et al., 2008 [PubMed 18449195]).[supplied by OMIM, Jun 2008]

Uniprot Description

OBFC2B: Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The SOSS complex associates with DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. Required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways. Component of the SOSS complex, composed of SOSS-B (SOSS- B1/OBFC2B or SOSS-B2/OBFC2A), SOSS-A/INTS3 and SOSS-C/C9orf80. SOSS complexes containing SOSS-B1/OBFC2B are more abundant than complexes containing SOSS-B2/OBFC2A. Directly interacts with ATM, SOSS-A/INTS3 and RAD51. Interacts with INTS7. Belongs to the SOSS-B family. SOSS-B1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; DNA repair, damage

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; single-stranded DNA binding

Biological Process: mitotic cell cycle checkpoint; response to ionizing radiation; DNA repair; response to DNA damage stimulus; double-strand break repair via homologous recombination