Rabbit anti-Human, Mouse NUP50 Polyclonal Antibody | anti-NUP50 antibody

NUP50, CT (Nucleoporin 50kD, NUP50 Protein, NUP50, HCG1989366, isoform CRA_c, CTA-268H5.7-002) (MaxLight 405)

Cat. Num. AAA6490401

• Gene Names: NUP50; NPAP60; NPAP60L
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: NUP50; Polyclonal Antibody; CT (Nucleoporin 50kD; NUP50 Protein; HCG1989366; isoform CRA_c; CTA-268H5.7-002) (MaxLight 405); anti-NUP50 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NUP50. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
• Sequence Length: 440

• Applicable Applications for anti-NUP50 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human NUP50 (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NUP50 (arrow) using rabbit polyclonal NUP50 Antibody (C-term) (RB08362). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUP50 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(Western blot analysis of anti-NUP50 Pab in mouse brain tissue lysate. NUP50(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-NUP50 antibody

Antibodies; Nuclear Proteins

References

1. Lindsay, M.E., et al., Cell 110(3):349-360 (2002). 2. Swaminathan, S., et al., Dev. Cell 3(3):304-306 (2002). 3. Smitherman, M., et al., Mol. Cell. Biol. 20(15):5631-5642 (2000). 4. Guan, T., et al., Mol. Cell. Biol. 20(15):5619-5630 (2000). 5. Collins, J.E., et al., Genome Biol. 5(10), R84 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 24497447
• NCBI GeneID: 10762
• NCBI Accession #: NP_705931
• NCBI GenBank Nucleotide #: NP_705931.1
• UniProt Accession #: Q8N6V5; Q9UKX7
• Molecular Weight: 46.863
• NCBI Official Full Name: nuclear pore complex protein Nup50 isoform a
• NCBI Official Synonym Full Names: nucleoporin 50
• NCBI Official Symbol: NUP50
• NCBI Official Synonym Symbols: NPAP60; NPAP60L
• NCBI Protein Information: nuclear pore complex protein Nup50
• UniProt Protein Name: Nuclear pore complex protein Nup50
• Protein Family: Nuclear pore complex protein
• UniProt Gene Name: NUP50
• UniProt Synonym Gene Names: NPAP60L
• UniProt Entry Name: NUP50_HUMAN

NCBI Description

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]