MesP1 recombinant protein

Human MesP1

Cat. Num. AAA691841

• Gene Names: MESP1; bHLHc5
• Reactivity: Human
• Purity: > 95% by SDS-PAGE & silver stain
• Synonyms: MesP1; Human MesP1; Recombinant Human MesP1; Mesoderm posterior protein 1; Class V basic helix-loop-helix protein 5; bHLHc5; MesP1 recombinant protein

• Host: Insect Cells
• Reactivity: Human
• Purity/Purification: > 95% by SDS-PAGE & silver stain
• Form/Format: Lyophilized
• Sequence: N-Terminal Sequence: UNDER WORK!
• Sequence Length: 268

• Buffer: 0.5X PBS
• Preparation and Storage: The lyophilized human MesP1, though stable at room temperature, is best stored desiccated below 0 degree C. Freeze/thaw cycles will result in significant loss of activity.

Testing Data

Related Product Information for MesP1 recombinant protein

ES-cell-based cardiovascular repair requires an in-depth understanding of the molecular mechanisms underlying the differentiation of cardiovascular ES cells. A candidate cardiovascular-fate inducer is the bHLH transcription factor MesP1 (1,2). As one of the earliest markers, it is expressed specifically in almost all cardiovascular precursors and is required for cardiac morphogenesis (2,3). It was shown that MesP1 is a key factor sufficient to induce the formation of ectopic heart tissue in vertebrates and increase cardiovasculogenesis by ES cells. Electrophysiological analysis showed all subtypes of cardiac ES-cell differentiation (4). MesP1 overexpression and knockdown experiments revealed a prominent function of MesP1 in a gene regulatory cascade, causing Dkk-1-mediated blockade of canonical Wnt-signalling. Independent evidence from ChIP and in vitro DNA-binding studies, expression analysis in wild-type and MesP knockout mice, and reporter assays confirm that Dkk-1 is a direct target of MesP1.

Product Categories/Family for MesP1 recombinant protein

Cytokines & Growth Factors

References

1. Saga Y et al, Development 126:3437, 1999; 2. Saga Y et al; Trends Cardiovasc Med 10:345, 2000; 3. Kitajima S et al, Dev Dyn 235:395, 2006; 4. Maltsev VA et al, Circ Res 75:233, 1994.

NCBI and Uniprot Product Information

• NCBI GI #: 14149724
• NCBI GeneID: 55897
• NCBI Accession #: NP_061140.1
• NCBI GenBank Nucleotide #: NM_018670
• UniProt Accession #: Q9BRJ9; Q9NSF1; Q9NSF2
• Molecular Weight: 34 kDa
• NCBI Official Full Name: mesoderm posterior protein 1
• NCBI Official Synonym Full Names: mesoderm posterior 1 homolog (mouse)
• NCBI Official Symbol: MESP1
• NCBI Official Synonym Symbols: bHLHc5
• NCBI Protein Information: mesoderm posterior protein 1; class C basic helix-loop-helix protein 5
• UniProt Protein Name: Mesoderm posterior protein 1
• Protein Family: Mesoderm posterior protein
• UniProt Gene Name: MESP1
• UniProt Synonym Gene Names: BHLHC5; bHLHc5
• UniProt Entry Name: MESP1_HUMAN

Uniprot Description

Function: Transcription factor. Plays a role in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. Defines the rostrocaudal patterning of the somites by participating in distinct Notch pathways

By similarity.

Subcellular location: Nucleus

Probable.

Miscellaneous: The N- and C-terminal domains are separated by a 2-repeat G-Q region.

Sequence similarities: Contains 1 bHLH (basic helix-loop-helix) domain.