Rabbit anti-Human NUP155 Polyclonal Antibody | anti-NUP155 antibody

NUP155, NT (Nuclear Pore Complex Protein Nup155, Nucleoporin Nup155, 155kD Nucleoporin, KIAA0791) (Biotin)

Cat. Num. AAA6490751

• Gene Names: NUP155; N155; ATFB15
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: NUP155; Polyclonal Antibody; NT (Nuclear Pore Complex Protein Nup155; Nucleoporin Nup155; 155kD Nucleoporin; KIAA0791) (Biotin); anti-NUP155 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NUP155.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-NUP155 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human NUP155 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NUP155 antibody (N-term) in HL60 cell line lysates (35ug/lane). NUP155 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

((LEFT)Formalin-fixed and paraffin-embedded human brain tissue reacted with NUP155 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT)Flow cytometric analysis of 293 cells using NUP155 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-NUP155 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

1. Zhang, X., Cell 135(6), 1017-1027 (2008). 2. Rayala, H.J., Mol. Cell Proteomics 3(2), 145-155 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 24430149
• NCBI GeneID: 9631
• NCBI Accession #: NP_705618
• NCBI GenBank Nucleotide #: NM_153485.2
• UniProt Accession #: O75694; Q9UBE9; Q9UFL5
• Molecular Weight: 149,016 Da
• NCBI Official Full Name: nuclear pore complex protein Nup155 isoform 1
• NCBI Official Synonym Full Names: nucleoporin 155kDa
• NCBI Official Symbol: NUP155
• NCBI Official Synonym Symbols: N155; ATFB15
• NCBI Protein Information: nuclear pore complex protein Nup155; nucleoporin 155kD; 155 kDa nucleoporin
• UniProt Protein Name: Nuclear pore complex protein Nup155
• Protein Family: Nuclear pore complex protein
• UniProt Gene Name: NUP155
• UniProt Synonym Gene Names: KIAA0791
• UniProt Entry Name: NU155_HUMAN

NCBI Description

Nucleoporins are proteins that play an important role in the assembly and functioning of the nuclear pore complex (NPC) which regulates the movement of macromolecules across the nuclear envelope (NE). The protein encoded by this gene plays a role in the fusion of NE vesicles and formation of the double membrane NE. The protein may also be involved in cardiac physiology and may be associated with the pathogenesis of atrial fibrillation. Alternative splicing results in multiple transcript variants of this gene. A pseudogene associated with this gene is located on chromosome 6. [provided by RefSeq, May 2013]

Uniprot Description

NUP155: Essential component of nuclear pore complex. Nucleoporins may be involved both in binding and translocating proteins during nucleocytoplasmic transport. Belongs to the non-repetitive/WGA-negative nucleoporin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleoporin

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: nuclear membrane; membrane; nuclear pore; nuclear envelope

Molecular Function: structural constituent of nuclear pore; transporter activity

Biological Process: viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; glucose transport; viral infectious cycle; nuclear membrane organization and biogenesis; mRNA export from nucleus; protein import into nucleus; hexose transport; carbohydrate metabolic process; viral transcription; gene expression; mitotic cell cycle; transmembrane transport

Disease: Atrial Fibrillation, Familial, 15