Rabbit anti-Human NUMBL Polyclonal Antibody | anti-NUMBL antibody

NUMBL, CT (NUMBL, Numb-like protein, Numb-related protein) (Biotin)

Cat. Num. AAA6327218

• Gene Names: NUMBL; NBL; CAG3A; CTG3a; NUMBR; NUMB-R; TNRC23; NUMBLIKE
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NUMBL; Polyclonal Antibody; CT (NUMBL; Numb-like protein; Numb-related protein) (Biotin); Numb-related protein; anti-NUMBL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-NUMBL antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NUMBL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-600 amino acids from the C-terminal region of human NUMBL.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis using MBS649100 in 293 cell line lysates (35ug/lane). This demonstrates the NUMBL antibody detected the NUMBL protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using MBS649100 in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Related Product Information for anti-NUMBL antibody

NUMBL plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF-kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons.

Product Categories/Family for anti-NUMBL antibody

Antibodies; Proteins

References

Zhou, L., et al. Biochem. Biophys. Res. Commun. 392(3):409-414(2010). Blom, T., et al. Cancer Genet. Cytogenet. 186(2):103-109(2008). Ma, Q., et al. Cell. Signal. 20(6):1044-1051(2008). Passos Gregorio, S., et al. Schizophr. Res. 88 (1-3), 275-282 (2006): Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 10863899
• NCBI GeneID: 9253
• NCBI Accession #: NP_004747.1
• NCBI GenBank Nucleotide #: NM_004756.4
• UniProt Accession #: Q9Y6R0; Q7Z4J9
• Molecular Weight: 64,891 Da
• NCBI Official Full Name: numb-like protein isoform a
• NCBI Official Synonym Full Names: numb homolog (Drosophila)-like
• NCBI Official Symbol: NUMBL
• NCBI Official Synonym Symbols: NBL; CAG3A; CTG3a; NUMBR; NUMB-R; TNRC23; NUMBLIKE
• NCBI Protein Information: numb-like protein; numb homolog-like; numb-related protein
• UniProt Protein Name: Numb-like protein
• Protein Family: Numb-like protein
• UniProt Gene Name: NUMBL
• UniProt Synonym Gene Names: Numb-R
• UniProt Entry Name: NUMBL_HUMAN

Uniprot Description

NUMBL: Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF- kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons. Interacts (via PTB domain) with MAP3K7IP2 (via C- terminal). Interacts (via C-terminal) with TRAF6 (via TRAF domains). Associates with EPS15 and NOTCH1.

Protein type: Cell development/differentiation

Chromosomal Location of Human Ortholog: 19q13.13-q13.2

Cellular Component: cytoplasm

Molecular Function: protein binding

Biological Process: nervous system development; positive regulation of neurogenesis; axonogenesis; cytokine and chemokine mediated signaling pathway; protein metabolic process; neuroblast division in the subventricular zone; positive regulation of dendrite morphogenesis; lateral ventricle development