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Western Blot (WB) (NUMB Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the NUMB antibody detected the NUMB protein (arrow).)

Rabbit anti-Human NUMB Polyclonal Antibody | anti-NUMB antibody

NUMB, NT (NUMB, Protein numb homolog, Protein S171) (APC)

Gene Names
NUMB; S171; C14orf41; c14_5527
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
NUMB; Polyclonal Antibody; NT (NUMB; Protein numb homolog; Protein S171) (APC); Protein S171; anti-NUMB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-NUMB antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
NUMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NUMB.
Conjugate
APC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NUMB Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the NUMB antibody detected the NUMB protein (arrow).)

Western Blot (WB) (NUMB Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the NUMB antibody detected the NUMB protein (arrow).)

Immunohistochemistry (IHC)

(NUMB Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NUMB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (NUMB Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NUMB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-NUMB antibody
The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-NUMB antibody
References
Rennstam, K., et al. Breast Cancer Res. Treat. 122(2):315-324(2010). Lu, C.B., et al. Neuroscience 161(2):403-412(2009). Chen, H., et al. Pathobiology 76(3):149-154(2009). Schluter, T., et al. Biochem. Biophys. Res. Commun. 379(4):909-913(2009). Kyriazis, G.A., et al. J. Biol. Chem. 283(37):25492-25502(2008).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
Molecular Weight
37,396 Da
NCBI Official Full Name
protein numb homolog isoform 3
NCBI Official Synonym Full Names
numb homolog (Drosophila)
NCBI Official Symbol
NUMB
NCBI Official Synonym Symbols
S171; C14orf41; c14_5527
NCBI Protein Information
protein numb homolog; h-Numb
UniProt Protein Name
Protein numb homolog
Protein Family
UniProt Gene Name
NUMB
UniProt Synonym Gene Names
h-Numb
UniProt Entry Name
NUMB_HUMAN

NCBI Description

The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NUMB: Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. Interacts with CDH1 and TFAP2B. Interacts with EPS15, LNX and NOTCH1. May interact with DUOXA1. Interacts with RALBP1 in a complex also containing EPN1 and TFAP2A during interphase and mitosis. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: extrinsic to plasma membrane; focal adhesion; clathrin-coated vesicle; apical part of cell; basolateral plasma membrane; integral to plasma membrane; early endosome; plasma membrane; nucleus

Molecular Function: protein binding; cadherin binding; beta-catenin binding; alpha-catenin binding

Biological Process: positive regulation of polarized epithelial cell differentiation; axon guidance; positive regulation of neurogenesis; Notch signaling pathway; negative regulation of Notch signaling pathway; neuroblast division in the subventricular zone; lateral ventricle development; positive regulation of cell migration

Research Articles on NUMB

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Product Notes

The NUMB numb (Catalog #AAA6327206) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NUMB, NT (NUMB, Protein numb homolog, Protein S171) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NUMB can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NUMB numb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUMB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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