Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using NUFIP1 antibody.)
Rabbit NUFIP1 Polyclonal Antibody | anti-NUFIP1 antibody
NUFIP1 Antibody
Gene Names
NUFIP1; NUFIP; bA540M5.1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NUFIP1; Polyclonal Antibody; NUFIP1 Antibody; bA540M5.1; Nuclear FMRP interacting protein 1; Nuclear fragile X mental retardation interacting protein 1; nuclear fragile X mental retardation protein interacting protein 1; NUFIP; anti-NUFIP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NUFIP1 antibody detects endogenous levels of NUFIP1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
495
Applicable Applications for anti-NUFIP1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human NUFIP1.
Subcellular Location
Nuclear
Tissue Specificity
Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (92%), Dog (100%), Xenopus (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using NUFIP1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using NUFIP1 antibody.)
Immunohistochemistry (IHC)
(MBS9603530 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603530 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-NUFIP1 antibody
Function: Binds RNA.
Subunit Structure: Interacts with FMR1 (PubMed:10556305). Interacts with ZNHIT3 (PubMed:28335020).
Subunit Structure: Interacts with FMR1 (PubMed:10556305). Interacts with ZNHIT3 (PubMed:28335020).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 70-75 kDa
Predicted: 57 kDa
Predicted: 57 kDa
NCBI Official Full Name
nuclear fragile X mental retardation-interacting protein 1
NCBI Official Synonym Full Names
nuclear FMR1 interacting protein 1
NCBI Official Symbol
NUFIP1
NCBI Official Synonym Symbols
NUFIP; bA540M5.1
NCBI Protein Information
nuclear fragile X mental retardation-interacting protein 1
UniProt Protein Name
Nuclear fragile X mental retardation-interacting protein 1
Protein Family
UniProt Gene Name
NUFIP1
NCBI Description
This gene encodes a nuclear RNA binding protein that contains a C2H2 zinc finger motif and a nuclear localization signal. This protein is associated with the nuclear matrix in perichromatin fibrils and, in neurons, localizes to the cytoplasm in association with endoplasmic reticulum ribosomes. This protein interacts with the fragile X mental retardation protein (FMRP), the tumor suppressor protein BRCA1, upregulates RNA polymerase II transcription, and is involved in box C/D snoRNP biogenesis. A pseudogene of this gene resides on chromosome 6q12. [provided by RefSeq, Feb 2012]
Uniprot Description
Binds RNA.
Research Articles on NUFIP1
Similar Products
Product Notes
The NUFIP1 nufip1 (Catalog #AAA9603530) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NUFIP1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NUFIP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the NUFIP1 nufip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUFIP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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