Western Blot (WB)
(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human normal liver tissue, Primary antibody: 861546(NUF2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Rabbit anti-Human Nuf2 Polyclonal Antibody | anti-NUF2 antibody
Nuf2 Rabbit pAb
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity purified
Synonyms
Nuf2; Polyclonal Antibody; Nuf2 Rabbit pAb; CDCA1; CT106; NUF2R; anti-NUF2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-NUF2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:200-1:1000
Immunogen
Synthetic peptide of human NUF2
Conjugation
Unconjugated
Molecular Function
This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation.
Modification
Unmodified
Cell Position
Centromere, Chromosome, Kinetochore, Nucleus
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human normal liver tissue, Primary antibody: 861546(NUF2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Western Blot (WB)
(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human normal liver tissue, Primary antibody: 861546(NUF2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Product Categories/Family for anti-NUF2 antibody
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
54kDa
UniProt Protein Name
Kinetochore protein Nuf2
Protein Family
UniProt Gene Name
NUF2
UniProt Synonym Gene Names
CDCA1; NUF2R; hNuf2; hNuf2R; hsNuf2
UniProt Entry Name
NUF2_HUMAN
Uniprot Description
NUF2: Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. Belongs to the NUF2 family.
Protein type: DNA replication; Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: 1q23.3
Cellular Component: membrane; nucleus; cytosol; chromosome, pericentric region
Molecular Function: protein binding
Biological Process: mitosis; cell division; mitotic cell cycle; chromosome segregation
Similar Products
Product Notes
The NUF2 nuf2 (Catalog #AAA4758408) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nuf2 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nuf2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:200-1:1000. Researchers should empirically determine the suitability of the NUF2 nuf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nuf2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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