Rabbit anti-Human NUDT2 Polyclonal Antibody | anti-NUDT2 antibody

NUDT2, ID (NUDT2, APAH1, Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical], Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase, Nucleoside diphosphate-linked moiety X motif 2) (Biotin)

Cat. Num. AAA6327141

• Gene Names: NUDT2; APAH1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NUDT2; Polyclonal Antibody; ID (NUDT2; APAH1; Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]; Diadenosine 5'; 5'''-P1; P4-tetraphosphate asymmetrical hydrolase; Nucleoside diphosphate-linked moiety X motif 2) (Biotin); Nucleoside diphosphate-linked moiety X motif 2; anti-NUDT2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-NUDT2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NUDT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from the Central region of human NUDT2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NUDT2 (arrow) using rabbit polyclonal NUDT2 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NUDT2 gene.)

Related Product Information for anti-NUDT2 antibody

This gene encodes a member of the MutT family of nucleotide pyrophosphatases, a subset of the larger NUDIX hydrolase family. The gene product possesses a modification of the MutT sequence motif found in certain nucleotide pyrophosphatases. The enzyme asymmetrically hydrolyzes Ap4A to yield AMP and ATP and is responsible for maintaining the intracellular level of the dinucleotide Ap4A, the function of which has yet to be established. This gene may be a candidate tumor suppressor gene. Alternative splicing has been observed at this locus and three transcript variants, all encoding the same protein, have been identified.

Product Categories/Family for anti-NUDT2 antibody

Antibodies; Enzymes; Phosphatase

References

Vasilenko, N., et al. Virol. J. 7, 31 (2010): Swarbrick, J.D., et al. J. Biol. Chem. 280(9):8471-8481(2005). Swarbrick, J.D., et al. J. Biomol. NMR 31(2):181-182(2005). Pitcher, W.H. III, et al. Antiviral Res. 58(3):227-233(2003). Guranowski, A., et al. Acta Biochim. Pol. 50(1):123-130(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 22219467
• NCBI GeneID: 318
• NCBI Accession #: NP_671702.1; NP_671701.1
• NCBI GenBank Nucleotide #: NM_147173.2
• UniProt Accession #: P50583; Q5T589; D3DRM0
• Molecular Weight: 16,829 Da
• NCBI Official Full Name: NUDT2 gene product
• NCBI Official Synonym Full Names: nudix hydrolase 2
• NCBI Official Symbol: NUDT2
• NCBI Official Synonym Symbols: APAH1
• NCBI Protein Information: bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]
• UniProt Protein Name: Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]
• Protein Family: Nudix hydrolase
• UniProt Gene Name: NUDT2
• UniProt Synonym Gene Names: APAH1; Ap4A hydrolase; Ap4Aase; Diadenosine tetraphosphatase; Nudix motif 2
• UniProt Entry Name: AP4A_HUMAN

NCBI Description

This gene encodes a member of the MutT family of nucleotide pyrophosphatases, a subset of the larger NUDIX hydrolase family. The gene product possesses a modification of the MutT sequence motif found in certain nucleotide pyrophosphatases. The enzyme asymmetrically hydrolyzes Ap4A to yield AMP and ATP and is responsible for maintaining the intracellular level of the dinucleotide Ap4A, the function of which has yet to be established. This gene may be a candidate tumor suppressor gene. Alternative splicing has been observed at this locus and four transcript variants, all encoding the same protein, have been identified. [provided by RefSeq, Sep 2011]

Uniprot Description

NUDT2: Asymmetrically hydrolyzes Ap4A to yield AMP and ATP. Plays a major role in maintaining homeostasis. Belongs to the Nudix hydrolase family.

Protein type: Hydrolase; Nucleotide Metabolism - pyrimidine; Apoptosis; Nucleotide Metabolism - purine; EC 3.6.1.17

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: mitochondrion

Molecular Function: GTP binding; bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity; bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity

Biological Process: apoptosis; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process