Immunocytochemistry/Immunofluorescence (ICC/IF))
Rabbit anti-Human Nucleostemin Polyclonal Antibody | anti-GNL3 antibody
Anti-Nucleostemin Antibody
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
Affinity purification. The antibody was purified by affinity chromatography
Synonyms
Nucleostemin; Polyclonal Antibody; Anti-Nucleostemin Antibody; E2IG3; NS; Guanine nucleotide-binding protein-like 3; E2-induced gene 3 protein; Novel nucleolar protein 47; NNP47; Nucleolar GTP-binding protein 3; anti-GNL3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Polyclonal antibody to detect endogenous levels of Nucleostemin in human samples
Purity/Purification
Affinity purification. The antibody was purified by affinity chromatography
Form/Format
Colorless liquid. In 0.42% Potassium phosphate; 0.87% Sodium chloride; pH7.3; 30% glycerol and 0.01% sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GNL3 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IF: 1:10-1:100
ICC: 1:10-1:100
IF: 1:10-1:100
ICC: 1:10-1:100
Immunogen
Recombinant full length protein of human Nucleostemin
Positive Control
WB: HEK293T cell lysates, IF: A549 cells
Preparation and Storage
Store at -20 degree C
The antibody solution should be gently mixed before use.
Shelf life: ~12 months
The antibody solution should be gently mixed before use.
Shelf life: ~12 months
Immunocytochemistry/Immunofluorescence (ICC/IF))
Immunocytochemistry/Immunofluorescence (ICC/IF))
Related Product Information for anti-GNL3 antibody
May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation.
Product Categories/Family for anti-GNL3 antibody
NCBI and Uniprot Product Information
NCBI GeneID
UniProt Accession #
UniProt Protein Name
Guanine nucleotide-binding protein-like 3
Protein Family
UniProt Gene Name
GNL3
UniProt Synonym Gene Names
E2IG3; NS
UniProt Entry Name
GNL3_HUMAN
Uniprot Description
GNL3: May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis. May interact with p53/TP53 via its basic domain. Increased levels in lung tissue in cancer patients. Belongs to the MMR1/HSR1 GTP-binding protein family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase; Cell cycle regulation; Nucleolus; RNA-binding
Chromosomal Location of Human Ortholog: 3p21.1
Cellular Component: nucleoplasm; extracellular space; membrane; nucleolus; nucleus
Molecular Function: GTPase activity; protein binding; GTP binding
Biological Process: cell proliferation; metabolic process; ribosome biogenesis and assembly; regulation of cell proliferation
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Product Notes
The Nucleostemin gnl3 (Catalog #AAA847508) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Nucleostemin Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleostemin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:1000 IF: 1:10-1:100 ICC: 1:10-1:100. Researchers should empirically determine the suitability of the Nucleostemin gnl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleostemin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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