Western Blot (WB)
(Western Blot: Sample: Human Jurket cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat NP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Rat Nucleoside Phosphorylase Polyclonal Antibody | anti-NP antibody
Polyclonal Antibody to Nucleoside Phosphorylase (NP)
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Human Jurket cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat NP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human Jurket cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat NP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue))
NCBI and Uniprot Product Information
NCBI Description
mouse purine-nucleoside phosphorylase (Pnp) exhibits purine-nucleoside phosphorylase and glycosyl transferase activities [RGD, Feb 2006]
Uniprot Description
NP: The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate
Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.4.2.1; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transferase
Chromosomal Location of Human Ortholog: 15p14
Cellular Component: cytoplasm; cytoskeleton; extracellular region; intracellular; nucleus
Molecular Function: drug binding; nucleoside binding; phosphate binding; purine binding; purine-nucleoside phosphorylase activity
Biological Process: immune response; inosine catabolic process; nicotinamide riboside catabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; positive regulation of alpha-beta T cell differentiation; positive regulation of T cell proliferation; purine nucleoside metabolic process; purine-containing compound salvage; response to drug
Research Articles on NP
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Product Notes
The NP pnp (Catalog #AAA2033901) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Nucleoside Phosphorylase (NP) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleoside Phosphorylase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the NP pnp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleoside Phosphorylase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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