Rabbit anti-Human Nucleoside Diphosphate Kinase C Polyclonal Antibody | anti-NDPKC antibody

Nucleoside Diphosphate Kinase C (NDPKC, NDPK-C, c371H6.2, DR-nm23, KIAA0516, Nucleoside Diphosphate Kinase 3, NDP Kinase 3, NDK 3, NDK3, NME3, NM23H3, nm23-H3) (FITC)

Cat. Num. AAA6490774

• Gene Names: NME3; NDPKC; NDPK-C; NM23H3; DR-nm23; NM23-H3; c371H6.2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Nucleoside Diphosphate Kinase C; Polyclonal Antibody; Nucleoside Diphosphate Kinase C (NDPKC; NDPK-C; c371H6.2; DR-nm23; KIAA0516; Nucleoside Diphosphate Kinase 3; NDP Kinase 3; NDK 3; NDK3; NME3; NM23H3; nm23-H3) (FITC); Non-metastatic Cells 3; Protein Expressed in; EC=2.7.4.6; anti-NDPKC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NME3.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NDPKC antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human NME3 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-NME3 Pab in CEM cell line tissue lysate (35ug/lane).NME3(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-NDPKC antibody

Antibodies; Protein Kinases

References

1. Negroni, A., et al., Cell Death Differ. 7(9):843-850 (2000). 2. Martinez, R., et al., Cancer Res. 57(6):1180-1187 (1997). 3. Venturelli, D., et al., Proc. Natl. Acad. Sci. USA 92(16):7435-7439 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 37693993
• NCBI GeneID: 4832
• NCBI Accession #: NP_002504
• NCBI GenBank Nucleotide #: NM_002513.2
• UniProt Accession #: Q13232; Q9BWH4
• Molecular Weight: 19.1 kDa (169aa), confirmed by MALDI-TOF
• NCBI Official Full Name: nucleoside diphosphate kinase 3
• NCBI Official Synonym Full Names: NME/NM23 nucleoside diphosphate kinase 3
• NCBI Official Symbol: NME3
• NCBI Official Synonym Symbols: NDPKC; NDPK-C; NM23H3; DR-nm23; NM23-H3; c371H6.2
• NCBI Protein Information: nucleoside diphosphate kinase 3
• UniProt Protein Name: Nucleoside diphosphate kinase 3
• UniProt Gene Name: NME3
• UniProt Synonym Gene Names: NDK 3; NDP kinase 3; NDPKC
• UniProt Entry Name: NDK3_HUMAN

Uniprot Description

NME3: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Probably has a role in normal hematopoiesis by inhibition of granulocyte differentiation and induction of apoptosis. Belongs to the NDK family.

Protein type: Kinase, other; Kinase, nucleoside diphosphate; Nucleotide Metabolism - pyrimidine; EC 2.7.4.6; Apoptosis; Motility/polarity/chemotaxis; Nucleotide Metabolism - purine; Other group; NDK family

Chromosomal Location of Human Ortholog: 16q13.3

Cellular Component: mitochondrion; cytosol

Molecular Function: metal ion binding; nucleoside diphosphate kinase activity; ATP binding

Biological Process: GTP biosynthetic process; regulation of apoptosis; CTP biosynthetic process; apoptosis; pyrimidine nucleotide metabolic process; UTP biosynthetic process; nucleoside triphosphate biosynthetic process; nucleoside diphosphate phosphorylation; purine nucleotide metabolic process