Human Matrix Metalloproteinase 28 ELISA Kit | MMP 28 elisa kit

Human Matrix Metalloproteinase 28 ELISA Kit

Cat. Num. AAA732957

• Gene Names: MMP28; MM28; MMP25; MMP-25; MMP-28; EPILYSIN
• Reactivity: Human
• Synonyms: Matrix Metalloproteinase 28; Human Matrix Metalloproteinase 28 ELISA Kit; MMP 28 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This MMP-28 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MMP-28. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP 28 elisa kit

Principle of the Assay: MMP-28 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MMP-28 antibody and an MMP-28-HRP conjugate. The assay sample and buffer are incubated together with MMP-28-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP-28 concentration since MMP-28 from samples and MMP-28-HRP conjugate compete for the anti-MMP-28 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP-28 from the sample, fewer sites are left to bind MMP-28-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP-28 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MMP 28 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 13236530
• NCBI GeneID: 79148
• NCBI Accession #: NP_077278.1
• NCBI GenBank Nucleotide #: NM_024302.4
• Molecular Weight: 14,927 Da
• NCBI Official Full Name: matrix metalloproteinase-28 isoform 1 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 28
• NCBI Official Symbol: MMP28
• NCBI Official Synonym Symbols: MM28; MMP25; MMP-25; MMP-28; EPILYSIN
• NCBI Protein Information: matrix metalloproteinase-28; matrix metalloprotease MMP25
• UniProt Protein Name: Matrix metalloproteinase-28
• UniProt Gene Name: MMP28
• UniProt Synonym Gene Names: MMP25; UNQ1893/PRO4339; MMP-28
• UniProt Entry Name: MMP28_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix for both normal physiological processes, such as embryonic development, reproduction and tissue remodeling, and disease processes, such as asthma and metastasis. This gene encodes a secreted enzyme that degrades casein. Its expression pattern suggests that it plays a role in tissue homeostasis and in wound repair. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

MMP28: Can degrade casein. Could play a role in tissues homeostasis and repair. Belongs to the peptidase M10A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; EC 3.4.24.-; Protease; Secreted

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: proteinaceous extracellular matrix; cytoplasm

Molecular Function: zinc ion binding; metalloendopeptidase activity; calcium ion binding

Biological Process: proteolysis