Goat Nucleoredoxin Polyclonal Antibody | anti-NXN antibody

Nucleoredoxin (NXN, FLJ12614, NRX, TRG-4, Red-1)

Cat. Num. AAA620834

• Gene Names: NXN; NRX; TRG-4; FLJ12614
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Nucleoredoxin; Polyclonal Antibody; Nucleoredoxin (NXN; FLJ12614; NRX; TRG-4; Red-1); Anti -Nucleoredoxin (NXN; anti-NXN antibody

• Host: Goat
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous human Nucleoredoxin at ~55kD by Western blot. Species Crossreactivity: Human, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute the antibody in 100ul PBS, 0.02% sodium azide. The antibody concentration will be 1mg/ml.

• Applicable Applications for anti-NXN antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1ug/ml
• Immunogen: Purified, E. coli-derived, recombinant human Nucleoredoxin (rhNRX; aa 131-435; Accession# Q6DKJ4).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-NXN antibody

Nucleoredoxin (NRX; also known as NXN and Red-1) is a 51-55kD member of the thioredoxin (TXR) family of proteins. It is widely expressed, and exists in both the nucleus and cytoplasm of cells where it regulates two pathways. In the nucleus, NRX may act as a transcription factor in the Wnt-pathway; a stable (reduced) NRX binds to DVL, thereby blocking downstream Wnt/ -catenin signaling. It also binds to PP2A subunits PP2A C and PP2A A, generating an active PP2A complex. Human NRX is 435 amino acids (aa) in length and contains a TXR domain (aa 167-321) that shows a WCPPC catalytic site, and a substrate recognition PDI-b' region (aa 312-427). There are three potential isoform variants. Over amino acids 131-435, human NRX shares 99% aa identity with mouse NRX.

Product Categories/Family for anti-NXN antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 33149331
• NCBI GeneID: 64359
• NCBI Accession #: NP_071908.2
• NCBI GenBank Nucleotide #: NM_022463.4
• UniProt Accession #: Q6DKJ4; Q3SWW6; Q6P3U6; Q9H9Q1; D3DTH2; Q56VW5; Q7L4C6
• Molecular Weight: 48,392 Da
• NCBI Official Full Name: nucleoredoxin isoform 1
• NCBI Official Synonym Full Names: nucleoredoxin
• NCBI Official Symbol: NXN
• NCBI Official Synonym Symbols: NRX; TRG-4; FLJ12614
• NCBI Protein Information: nucleoredoxin; nucleoredoxin 1; OTTHUMP00000115356
• UniProt Protein Name: Nucleoredoxin
• Protein Family: Nucleoredoxin
• UniProt Gene Name: NXN
• UniProt Synonym Gene Names: NRX
• UniProt Entry Name: NXN_HUMAN

Uniprot Description

NXN: Functions as a redox-dependent negative regulator of the Wnt signaling pathway, possibly by preventing ubiquitination of DVL3 by the BCR(KLHL12) complex. May also function as a transcriptional regulator act as a regulator of protein phosphatase 2A (PP2A). Belongs to the nucleoredoxin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.8.1.8; Oxidoreductase

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: nucleus; cytosol

Molecular Function: protein-disulfide reductase activity; thioredoxin-disulfide reductase activity

Biological Process: negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; negative regulation of protein ubiquitination; cell differentiation