Western Blot (WB)
(Western blot analysis of Nucleophosmin expression in A549 (A), LNCaP (B), HeLa (C) whole cell lysates.)
Rabbit Nucleophosmin Polyclonal Antibody | anti-NPM1 antibody
Anti-Nucleophosmin Antibody
Western Blot (WB)
(Western blot analysis of Nucleophosmin expression in A549 (A), LNCaP (B), HeLa (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Nucleophosmin expression in A549 (A), LNCaP (B), HeLa (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Nucleophosmin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Nucleophosmin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]
Uniprot Description
NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.
Protein type: Translation; Nucleolus; RNA-binding; Oncoprotein
Chromosomal Location of Human Ortholog: 5q35.1
Cellular Component: nucleoplasm; centrosome; focal adhesion; membrane; cytoplasm; nucleolus; spindle pole centrosome; cytosol; ribonucleoprotein complex; nucleus
Molecular Function: protein homodimerization activity; protein kinase inhibitor activity; histone binding; RNA binding; unfolded protein binding; transcription coactivator activity; protein kinase binding; ribosomal large subunit binding; NF-kappaB binding; protein binding; Tat protein binding; protein heterodimerization activity; ribosomal small subunit binding
Biological Process: regulation of centriole replication; positive regulation of translation; viral reproduction; centrosome cycle; cell aging; nucleocytoplasmic transport; DNA repair; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; nucleosome assembly; intracellular protein transport; regulation of endodeoxyribonuclease activity; protein localization; DNA replication-independent nucleosome assembly at centromere; ribosome assembly; response to stress; negative regulation of apoptosis
Research Articles on NPM1
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Product Notes
The NPM1 npm1 (Catalog #AAA820466) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Nucleophosmin Antibody reacts with Human, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleophosmin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100). Researchers should empirically determine the suitability of the NPM1 npm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleophosmin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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