Western Blot (WB)
(Western blot analysis of Nucleobindin-2 in rat brain tissue lysate with Nucleobindin-2 antibody at (A) 0.5 and (B) 1 μg/mL.)
Rabbit Nucleobindin-2 Polyclonal Antibody | anti-NUCB2 antibody
Nucleobindin-2 Antibody
Gene Names
NUCB2; NEFA; HEL-S-109
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Nucleobindin-2 Antibody is affinity chromatography purified via peptide column.
Synonyms
Nucleobindin-2; Polyclonal Antibody; Nucleobindin-2 Antibody; NEFA; HEL-S-109; DNA-binding protein NEFA; nucleobindin 2; anti-NUCB2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Nucleobindin-2 antibody is predicted to not cross-react with NUCB1.
Purity/Purification
Nucleobindin-2 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
420
Applicable Applications for anti-NUCB2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Nucleobindin-2 Antibody can be used for detection of Nucleobindin-2 by Western blot starting at 0.5 mug/mL.
Conjugate
Unconjugated
Immunogen
Nucleobindin-2 antibody was raised against a 16 amino acid peptide near the center of human Nucleobindin-2.
Buffer
Nucleobindin-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
Nucleobindin-2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of Nucleobindin-2 in rat brain tissue lysate with Nucleobindin-2 antibody at (A) 0.5 and (B) 1 μg/mL.)
Western Blot (WB)
(Western blot analysis of Nucleobindin-2 in rat brain tissue lysate with Nucleobindin-2 antibody at (A) 0.5 and (B) 1 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of Nucleobindin-2 in rat brain tissue with Nucleobindin-2 antibody at 2.5 μg/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry of Nucleobindin-2 in rat brain tissue with Nucleobindin-2 antibody at 2.5 μg/ml.)
Immunofluorescence (IF)
(Immunofluorescence of Nucleobindin-2 in rat brain tissue with Nucleobindin-2 antibody at 20 μg/ml.)
Immunofluorescence (IF)
(Immunofluorescence of Nucleobindin-2 in rat brain tissue with Nucleobindin-2 antibody at 20 μg/ml.)
Related Product Information for anti-NUCB2 antibody
Nucleobindin-2 Antibody: NUCB2 was initially identified as calcium-binding protein EF hand motif-containing protein that could bind with Necdin, a growth suppressor expressed primarily in postmitotic neurons. NUC2 is a secreted protein that is cleaved into three major peptide products, Nesfatin-1, Nesfatin-2, and Nesfatin-3, of which Nesfatin-1 has been found to cause the suppression of food intake in a leptin-independent manner. Other studies have suggested that NUCB2/Nesfatin-1 may also play roles in energy homeostasis and closely related neuroendocrine functions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 46 kDa
Observed: 54 kDa
Observed: 54 kDa
NCBI Official Full Name
nucleobindin-2
NCBI Official Synonym Full Names
nucleobindin 2
NCBI Official Symbol
NUCB2
NCBI Official Synonym Symbols
NEFA; HEL-S-109
NCBI Protein Information
nucleobindin-2; nesfatin 1; nesfatin-1; prepronefastin; prepronesfatin; nucleobinding 2; DNA-binding protein NEFA; gastric cancer antigen Zg4; epididymis secretory protein Li 109
UniProt Protein Name
Nucleobindin-2
Protein Family
UniProt Gene Name
NUCB2
UniProt Synonym Gene Names
NEFA
UniProt Entry Name
NUCB2_HUMAN
NCBI Description
This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]
Uniprot Description
NUCB2: Calcium-binding protein. May have a role in calcium homeostasis. Belongs to the nucleobindin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Vesicle; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11p15.1
Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum; plasma membrane; ER-Golgi intermediate compartment; nuclear envelope; cytosol
Molecular Function: protein binding; DNA binding; calcium ion binding
Research Articles on NUCB2
Similar Products
Product Notes
The NUCB2 nucb2 (Catalog #AAA153535) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nucleobindin-2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleobindin-2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Nucleobindin-2 Antibody can be used for detection of Nucleobindin-2 by Western blot starting at 0.5 mug/mL. Researchers should empirically determine the suitability of the NUCB2 nucb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleobindin-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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