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Western Blot (WB) (Western BlotPositive WB detected in: Rat brain tissue,Rat heart tissue,Mouse heart tissue,Mouse liver tissue,Mouse brain tissueAll lanes: NUCB2 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 51 KDaObserved band size: 51 KDa)

Rabbit NUCB2 Polyclonal Antibody | anti-NUCB2 antibody

NUCB2 Antibody

Gene Names
NUCB2; NEFA; HEL-S-109
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
>95%, Protein G purified
Synonyms
NUCB2; Polyclonal Antibody; NUCB2 Antibody; Nucleobindin-2; DNA-binding protein NEFA; Gastric cancer antigen Zg4; Prepronesfatin; Nesfatin-1; NEFA; anti-NUCB2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
420
Applicable Applications for anti-NUCB2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:5000
IHC: 1:20-1:200
Immunogen
Recombinant Human Nucleobindin-2 protein (25-106AA)
Conjugation
Non-conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Rat brain tissue,Rat heart tissue,Mouse heart tissue,Mouse liver tissue,Mouse brain tissueAll lanes: NUCB2 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 51 KDaObserved band size: 51 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: Rat brain tissue,Rat heart tissue,Mouse heart tissue,Mouse liver tissue,Mouse brain tissueAll lanes: NUCB2 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 51 KDaObserved band size: 51 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate cancer using MBS7052201 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human prostate cancer using MBS7052201 at dilution of 1:100)
Related Product Information for anti-NUCB2 antibody
Calcium-binding protein. May have a role in calcium homeostasis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,201 Da
NCBI Official Full Name
nucleobindin-2 isoform 1
NCBI Official Synonym Full Names
nucleobindin 2
NCBI Official Symbol
NUCB2
NCBI Official Synonym Symbols
NEFA; HEL-S-109
NCBI Protein Information
nucleobindin-2
UniProt Protein Name
Nucleobindin-2
Protein Family
UniProt Gene Name
NUCB2
UniProt Synonym Gene Names
NEFA

NCBI Description

This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]

Uniprot Description

NUCB2: Calcium-binding protein. May have a role in calcium homeostasis. Belongs to the nucleobindin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Vesicle

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: cytosol; ER-Golgi intermediate compartment; extracellular space; plasma membrane

Molecular Function: protein binding

Research Articles on NUCB2

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Product Notes

The NUCB2 nucb2 (Catalog #AAA7052201) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NUCB2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NUCB2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:5000 IHC: 1:20-1:200. Researchers should empirically determine the suitability of the NUCB2 nucb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUCB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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