Immunohistochemistry (IHC)
(Immunochemical staining of human ENO2 in human pancreas with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
Rabbit anti-Human NSE/ENO2 Polyclonal Antibody | anti-ENO2 antibody
Anti-NSE/ENO2 Antibody, Rabbit Polyclonal
IP: 1-4uL/mg of lysate
Immunohistochemistry (IHC)
(Immunochemical staining of human ENO2 in human pancreas with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human ENO2 in human pancreas with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human ENO2 in human brain with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human ENO2 in human brain with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
Immunoprecipitation (IP)
(ENO2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-ENO2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ENO2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 47 kDaObserved band size: 47 kDa)
Immunoprecipitation (IP)
(ENO2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-ENO2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ENO2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 47 kDaObserved band size: 47 kDa)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
Uniprot Description
ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; EC 4.2.1.11
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: extracellular space; photoreceptor inner segment; phosphopyruvate hydratase complex; plasma membrane; perikaryon; cytosol
Molecular Function: magnesium ion binding; phosphopyruvate hydratase activity
Biological Process: glycolysis; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis
Research Articles on ENO2
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Product Notes
The ENO2 eno2 (Catalog #AAA8106848) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NSE/ENO2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NSE/ENO2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). IHC-P: 1:10,000-1:20,000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the ENO2 eno2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NSE/ENO2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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