Rabbit NSD3 Polyclonal Antibody | anti-NSD3 antibody

NSD3 Polyclonal Antibody

Cat. Num. AAA3013154

• Gene Names: NSD3; KMT3F; KMT3G; WHISTLE; WHSC1L1; pp14328
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: NSD3; Polyclonal Antibody; NSD3 Polyclonal Antibody; WHSC1-like protein 1; WHSC1L1; DKFZp667H044; FLJ20353; Histone lysine N methyltransferase NSD3; Histone-lysine N-methyltransferase NSD3; MGC126766; MGC142029; NSD 3; Nuclear set domain containing 3; Nuclear SET domain containing protein 3; Nuclear SET domain-containing protein 3; pp14328; Protein whistle; Whistle; WHSC1 like protein 1; WHSC1-like 1 isoform 9 with methyltransferase activity to lysine; WHSC1L1 protein; Wolf Hirschhorn syndrome candidate 1 like 1; Wolf-Hirschhorn syndrome candidate 1-like protein 1; anti-NSD3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NSD3 polyclonal antibody detects endogenous levels of NSD3 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1437

• Applicable Applications for anti-NSD3 antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1,000-1:2,000; FC/FACS: 1:50-1:100
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of NSD3 on different lysates using anti-NSD3 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: 293T)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Hela cells with NSD3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

Related Product Information for anti-NSD3 antibody

The deduced 1, 437 amino acid NSD3 protein contains two PWWP domains involved in protein-protein interactions, five PHD-type zinc finger motifs found in chromatin-associated proteins, a SAC (SET-associated cys-rich) domain, a SET domain and a C-terminal C5HCH domain. Two NSD3 variants have been identified. The short variant comprised of 645 amino acids, arises from alternative polyadenylation and exon splicing and contains a single PWWP domain. A longer NSD3 variant, which is only expressed in HeLa cells, is comprised of 1, 388 amino acid residues. The human WHSC1L1 gene, which encodes the NSD3 protein, shares 68% and 55% identity with mouse Nsd1 and human WHSC1, respectively. Highest expression of NSD3 is observed in brain, heart and skeletal muscle tissues; lower levels of NSD3 expression are observed in the liver and lungs.

NCBI and Uniprot Product Information

• NCBI GI #: 74761342
• NCBI GeneID: 54904
• UniProt Accession #: Q9BZ95; Q6P2L6
• Molecular Weight: 162kDa
• NCBI Official Full Name: Histone-lysine N-methyltransferase NSD3
• NCBI Official Synonym Full Names: nuclear receptor binding SET domain protein 3
• NCBI Official Symbol: NSD3
• NCBI Official Synonym Symbols: KMT3F; KMT3G; WHISTLE; WHSC1L1; pp14328
• NCBI Protein Information: histone-lysine N-methyltransferase NSD3
• UniProt Protein Name: Histone-lysine N-methyltransferase NSD3
• UniProt Gene Name: WHSC1L1
• UniProt Synonym Gene Names: NSD3; WHSC1-like protein 1
• UniProt Entry Name: NSD3_HUMAN

NCBI Description

This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described. [provided by RefSeq, May 2015]

Uniprot Description

WHSC1L1: is related to the Wolf-Hirschhorn syndrome candidate-1 protein containing PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced isoforms have been described.

Protein type: Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine; Oncoprotein; Histone-binding; EC 2.1.1.43; Methyltransferase

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: nucleoplasm; chromosome; nucleus

Molecular Function: protein binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific)

Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; histone H3-K4 methylation; negative regulation of transcription, DNA-dependent

Disease: Leukemia, Acute Myeloid