Rabbit anti-Human, Mouse NSD1 Polyclonal Antibody | anti-NSD1 antibody

NSD1 Polyclonal Antibody

Cat. Num. AAA3009370

• Gene Names: NSD1; STO; KMT3B; SOTOS; ARA267; SOTOS1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NSD1; Polyclonal Antibody; NSD1 Polyclonal Antibody; ARA267; KMT3B; H3-K36-HMTase; H4-K20-HMTase; Lysine N-methyltransferase 3B; anti-NSD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NSD1 polyclonal antibody detects endogenous levels of NSD1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 2696

• Applicable Applications for anti-NSD1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Recombinant protein of human NSD1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cells, using NSD1 antibody.)

Related Product Information for anti-NSD1 antibody

The nuclear receptor-binding SET domain-containing protein 1 (NSD1) belongs to a family of proteins which have all been implicated in human malignancy. The protein family includes NSD2 and NSD3, both of which show 70-75% sequence identity with NSD1 but contribute substantially less to overgrowth phenotypes. Defects and microdeletions of the NSD1 gene are involved in Sotos syndrome, childhood acute myeloid leukemia (AML), Weaver syndrome and Beckwith-Wiedemann Syndrome (BWS). The protein functions as a transcriptional intermediary factor capable of influencing transcription, either negatively or positively, depending on the cellular context. NSD1 is a nuclear protein expressed in brain, muscle, spleen, thymus, kidney and, to a lesser extent, lung.

NCBI and Uniprot Product Information

• NCBI GI #: 19923586
• NCBI GeneID: 64324
• NCBI Accession #: NP_071900
• NCBI GenBank Nucleotide #: NP_071900.2
• UniProt Accession #: Q96L73
• Molecular Weight: ~ 297kDa
• NCBI Official Full Name: histone-lysine N-methyltransferase, H3 lysine-36 specific isoform b
• NCBI Official Synonym Full Names: nuclear receptor binding SET domain protein 1
• NCBI Official Symbol: NSD1
• NCBI Official Synonym Symbols: STO; KMT3B; SOTOS; ARA267; SOTOS1
• NCBI Protein Information: histone-lysine N-methyltransferase, H3 lysine-36 specific; histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific
• UniProt Protein Name: Histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: NSD1
• UniProt Synonym Gene Names: ARA267; KMT3B
• UniProt Entry Name: NSD1_HUMAN

NCBI Description

This gene encodes a protein containing a SET domain, 2 LXXLL motifs, 3 nuclear translocation signals (NLSs), 4 plant homeodomain (PHD) finger regions, and a proline-rich region. The encoded protein enhances androgen receptor (AR) transactivation, and this enhancement can be increased further in the presence of other androgen receptor associated coregulators. This protein may act as a nucleus-localized, basic transcriptional factor and also as a bifunctional transcriptional regulator. Mutations of this gene have been associated with Sotos syndrome and Weaver syndrome. One version of childhood acute myeloid leukemia is the result of a cryptic translocation with the breakpoints occurring within nuclear receptor-binding Su-var, enhancer of zeste, and trithorax domain protein 1 on chromosome 5 and nucleoporin, 98-kd on chromosome 11. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Sep 2018]