Rabbit CCR3 Polyclonal Antibody | anti-CCR3 antibody

CCR3 Antibody

Cat. Num. AAA150409

• Gene Names: CCR3; CKR3; CD193; CMKBR3; CC-CKR-3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: CCR3 Antibody is immunoaffinity purified IgG.
• Synonyms: CCR3; Polyclonal Antibody; CCR3 Antibody; CKR3; CD193; CMKBR3; CC-CKR-3; C-C chemokine receptor type 3; Eosinophil eotaxin receptor; C-C CKR-3; chemokine (C-C motif) receptor 3; anti-CCR3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: CCR3 Antibody is immunoaffinity purified IgG.
• Form/Format: Liquid
• Sequence Length: 355

• Applicable Applications for anti-CCR3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: CCR3 antibody can be used for the detection of CCR3 by Western blot at 1 - 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: CCR3 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human CCR3.
• Buffer: CCR3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CCR3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CCR3 in human spleen tissue lysates with CCR3 antibody at 1 (lane 1) and 2 μg/mL (lane 2), and 2 μg/mL in the presence of blocking peptide (lane 3). )

Related Product Information for anti-CCR3 antibody

CCR3 Antibody: Human immunodeficiency virus (HIV) and related virus require coreceptors to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b, CCR8, GPR15, STRL33, and CX3CR1 in the chemokine receptor family were recently identified as HIV coreceptors. CCR5, CXCR4 and CCR3 are the principal receptors for HIV fusion and entry of target cells. CCR3 facilitates infection by a subset of virus. CCR3 and CCR5 promote efficient infection of microglia, the major target cells in the CNS. High levels of CCR3 and CXCR4 expression were found on the neurons from both the central and peripheral nervous systems. The CCR3 ligand, eotaxin, and an anti-CCR3 antibody inhibited HIV infection of microglia. These results indicate CCR3 plays an important role in HIV infection of CNS.

NCBI and Uniprot Product Information

• NCBI GI #: 30581170
• NCBI GeneID: 1232
• NCBI Accession #: NP_847899
• NCBI GenBank Nucleotide #: NM_178329.2
• UniProt Accession #: P51677; Q15748; Q2YDB9; Q86WD2; Q8TDP6; Q9ULY8; B3KVQ1; F5GWL6
• Molecular Weight: 43,453 Da
• NCBI Official Full Name: C-C chemokine receptor type 3 isoform 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) receptor 3
• NCBI Official Symbol: CCR3
• NCBI Official Synonym Symbols: CKR3; CD193; CMKBR3; CC-CKR-3
• NCBI Protein Information: C-C chemokine receptor type 3; CCR-3; C-C CKR-3; b-chemokine receptor; CC chemokine receptor 3; eosinophil eotaxin receptor; eosinophil CC chemokine receptor 3
• UniProt Protein Name: C-C chemokine receptor type 3
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR3
• UniProt Synonym Gene Names: CMKBR3; C-C CKR-3; CC-CKR-3; CCR-3; CCR3; CKR3
• UniProt Entry Name: CCR3_HUMAN

NCBI Description

The protein encoded by this gene is a receptor for C-C type chemokines. It belongs to family 1 of the G protein-coupled receptors. This receptor binds and responds to a variety of chemokines, including eotaxin (CCL11), eotaxin-3 (CCL26), MCP-3 (CCL7), MCP-4 (CCL13), and RANTES (CCL5). It is highly expressed in eosinophils and basophils, and is also detected in TH1 and TH2 cells, as well as in airway epithelial cells. This receptor may contribute to the accumulation and activation of eosinophils and other inflammatory cells in the allergic airway. It is also known to be an entry co-receptor for HIV-1. This gene and seven other chemokine receptor genes form a chemokine receptor gene cluster on the chromosomal region 3p21. Alternatively spliced transcript variants have been described. [provided by RefSeq, Sep 2009]

Uniprot Description

CCR3: Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; C-C chemokine receptor activity; chemokine receptor activity

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; elevation of cytosolic calcium ion concentration; positive regulation of angiogenesis; viral reproduction; cellular defense response; positive regulation of endothelial cell proliferation; chemotaxis; inflammatory response; cell adhesion