Rabbit NRP1 Polyclonal Antibody | anti-NRP1 antibody

NRP1 Antibody

Cat. Num. AAA9402825

• Gene Names: NRP1; NP1; NRP; BDCA4; CD304; VEGF165R
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: NRP1; Polyclonal Antibody; NRP1 Antibody; NP1; NRP; BDCA4; CD304; VEGF165R; anti-NRP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total NRP1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 644

• Applicable Applications for anti-NRP1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human NRP1.
• Target Name: NRP1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using NRP1 antibody.)

Related Product Information for anti-NRP1 antibody

This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contains a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.

Product Categories/Family for anti-NRP1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 66912178
• NCBI GeneID: 8829
• NCBI Accession #: NP_001019799.1
• NCBI GenBank Nucleotide #: NM_001024628.2
• UniProt Accession #: O14786; O60461; Q5T7F1; Q5T7F2; Q5T7F3; Q86T59; Q96I90; Q96IH5; B0LPG9
• Molecular Weight: 68,376 Da
• NCBI Official Full Name: neuropilin-1 isoform b
• NCBI Official Synonym Full Names: neuropilin 1
• NCBI Official Symbol: NRP1
• NCBI Official Synonym Symbols: NP1; NRP; BDCA4; CD304; VEGF165R
• NCBI Protein Information: neuropilin-1
• UniProt Protein Name: Neuropilin-1
• Protein Family: Neuropilin
• UniProt Gene Name: NRP1
• UniProt Synonym Gene Names: NRP; VEGF165R
• UniProt Entry Name: NRP1_HUMAN

NCBI Description

This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contains a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

NRP1: The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. Homodimer, and heterodimer with NRP2. Interacts with FER. Binds PLXNB1. The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. Belongs to the neuropilin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 10p12

Cellular Component: extracellular space; cell surface; growth cone; focal adhesion; cell soma; axon; early endosome; neurofilament; plasma membrane; integral to membrane; cytoplasmic vesicle; cytosol; receptor complex

Molecular Function: heparin binding; vascular endothelial growth factor receptor activity; protein binding; semaphorin receptor activity; cytokine binding; metal ion binding; growth factor binding; coreceptor activity

Biological Process: axon guidance; facial nerve structural organization; neuron migration; vestibulocochlear nerve structural organization; platelet-derived growth factor receptor signaling pathway; signal transduction; negative regulation of axon extension involved in axon guidance; positive regulation of smooth muscle cell migration; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; cell-cell signaling; positive chemotaxis; branchiomotor neuron axon guidance; response to wounding; dendrite development; angiogenesis; negative regulation of neuron apoptosis; trigeminal nerve structural organization; axonal fasciculation; hepatocyte growth factor receptor signaling pathway; patterning of blood vessels; axon extension involved in axon guidance; organ morphogenesis; positive regulation of peptidyl-tyrosine phosphorylation; cell migration during sprouting angiogenesis; otic placode formation; positive regulation of axon extension involved in axon guidance; artery morphogenesis; positive regulation of endothelial cell proliferation; nerve development; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; retinal ganglion cell axon guidance