Western Blot (WB)
(NRK Antibody (Center) (MBS9204967) western blot analysis in A549 cell line lysates (35ug/lane). This demonstrates the NRK antibody detected the NRK protein (arrow).)
Rabbit anti-Human NRK Polyclonal Antibody | anti-NRK antibody
NRK Antibody (Center)
IF~~1:10~50
Western Blot (WB)
(NRK Antibody (Center) (MBS9204967) western blot analysis in A549 cell line lysates (35ug/lane). This demonstrates the NRK antibody detected the NRK protein (arrow).)
Western Blot (WB)
(NRK Antibody (Center) (MBS9204967) western blot analysis in A549 cell line lysates (35ug/lane). This demonstrates the NRK antibody detected the NRK protein (arrow).)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with NRK Antibody (Center) (MBS9204967). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NRK primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NRK immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with NRK Antibody (Center) (MBS9204967). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NRK primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NRK immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
Nakano, K., et al. Exp. Cell Res. 287(2):219-227(2003)
NCBI and Uniprot Product Information
NCBI Description
The mouse ortholog of this gene encodes a protein kinase required for JNK activation. The encoded protein may be involved in the induction of actin polymerization in late embryogenesis.[provided by RefSeq, Jun 2010]
Uniprot Description
NRK: May phosphorylate cofilin-1 and induce actin polymerization through this process, during the late stages of embryogenesis. Involved in the TNF-alpha-induced signaling pathway. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; EC 2.7.11.1; Kinase, protein; STE group; STE20 family; MSN subfamily
Chromosomal Location of Human Ortholog: Xq22.3
Cellular Component: cytoplasm
Molecular Function: ATP binding; receptor signaling protein serine/threonine kinase activity
Biological Process: negative regulation of cell proliferation; parturition; protein amino acid phosphorylation; activation of JNKK activity
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Product Notes
The NRK nrk (Catalog #AAA9204967) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 721-750. The NRK Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NRK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB),Immunofluorescence (IF), ELISA (EIA). WB~~1:1000 IF~~1:10~50. Researchers should empirically determine the suitability of the NRK nrk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NRK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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