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Western Blot (WB) (Fig.1. Western Blot analysis of A549 cells using Acetyl-Nrf2 (K599) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary Antibody was diluted at 1:20000.)

Rabbit Nrf2 Polyclonal Antibody | anti-NRF2 antibody

Nrf2 (Acetyl Lys599) Polyclonal Antibody

Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Nrf2; Polyclonal Antibody; Nrf2 (Acetyl Lys599) Polyclonal Antibody; Rabbit Anti-Nrf2 (Acetyl Lys599) Polyclonal Antibody; NFE2L2; NRF2; Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor; erythroid derived 2; like 2; anti-NRF2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Acetyl-Nrf2 (K599) Polyclonal Antibody detects endogenous levels of Nrf2 protein only when non-acetylation at K599.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
589
Applicable Applications for anti-NRF2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:100-1:300
ELISA: 1:10000
Immunogen
Synthesized peptide derived from the C-terminal region of human Nrf2 around the acetylation site of K599
Preparation and Storage
Stable for one year at -20 degree C from date of shipment.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Western Blot (WB)

(Fig.1. Western Blot analysis of A549 cells using Acetyl-Nrf2 (K599) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary Antibody was diluted at 1:20000.)

Western Blot (WB) (Fig.1. Western Blot analysis of A549 cells using Acetyl-Nrf2 (K599) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary Antibody was diluted at 1:20000.)

Immunohistochemistry (IHC)

(Fig.2. Immunohistochemical analysis of paraffin-embedded rat-lung, antibody was diluted at 1:100.)

Immunohistochemistry (IHC) (Fig.2. Immunohistochemical analysis of paraffin-embedded rat-lung, antibody was diluted at 1:100.)

Immunohistochemistry (IHC)

(Fig.3. Immunohistochemical analysis of paraffin-embedded rat-lung, antibody was diluted at 1:100.)

Immunohistochemistry (IHC) (Fig.3. Immunohistochemical analysis of paraffin-embedded rat-lung, antibody was diluted at 1:100.)

Immunohistochemistry (IHC)

(Fig.4. Immunohistochemical analysis of paraffin-embedded rat-lung, antibody was diluted at 1:100.)

Immunohistochemistry (IHC) (Fig.4. Immunohistochemical analysis of paraffin-embedded rat-lung, antibody was diluted at 1:100.)

Immunohistochemistry (IHC)

(Fig.5. Immunohistochemical analysis of paraffin-embedded mouse-lung, antibody was diluted at 1:100.)

Immunohistochemistry (IHC) (Fig.5. Immunohistochemical analysis of paraffin-embedded mouse-lung, antibody was diluted at 1:100.)

Immunohistochemistry (IHC)

(Fig.6. Immunohistochemical analysis of paraffin-embedded mouse-lung, antibody was diluted at 1:100.)

Immunohistochemistry (IHC) (Fig.6. Immunohistochemical analysis of paraffin-embedded mouse-lung, antibody was diluted at 1:100.)

Immunohistochemistry (IHC)

(Fig.7. Immunohistochemical analysis of paraffin-embedded mouse-lung, antibody was diluted at 1:100.)

Immunohistochemistry (IHC) (Fig.7. Immunohistochemical analysis of paraffin-embedded mouse-lung, antibody was diluted at 1:100.)
Related Product Information for anti-NRF2 antibody
NFE2L2 encodes a transcription factor (nuclear factor, erythroid 2 like 2) which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for NFE2L2.

NCBI and Uniprot Product Information

NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
nuclear factor erythroid 2-related factor 2 isoform 2
UniProt Protein Name
Nuclear factor erythroid 2-related factor 2
UniProt Gene Name
NFE2L2
UniProt Synonym Gene Names
NRF2; NF-E2-related factor 2
UniProt Entry Name
NF2L2_HUMAN

Uniprot Description

NRF2: a transcription factor that regulates basal expression and antioxidant induction of a transcription factor for NAD(P)H:quinone oxidoreductase-1 (NQO1) and other detoxifying genes. Targeted for proteasomal degradation by INrf2. Oxidative stress causes its phosphorylation and release from INrf2.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: nucleoplasm; centrosome; cytoplasm; plasma membrane; nucleus; chromatin; cytosol

Molecular Function: protein domain specific binding; protein binding; DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; positive regulation of blood coagulation; unfolded protein response; regulation of embryonic development; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter

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Product Notes

The NRF2 nfe2l2 (Catalog #AAA9700910) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nrf2 (Acetyl Lys599) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Nrf2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). WB: 1:500-1:2000 IHC-P: 1:100-1:300 ELISA: 1:10000. Researchers should empirically determine the suitability of the NRF2 nfe2l2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nrf2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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