Human Plasma Renin Activity ELISA Kit | PRA elisa kit

Plasma Renin Activity (PRA) ELISA

• Reactivity: Human
• Synonyms: Plasma Renin Activity; Plasma Renin Activity (PRA) ELISA; PRA elisa kit

• Reactivity: Human

• Assay Type: Quantitative Competitive
• Samples: Human EDTA plasma by an ELISA (Enzyme-Linked Immunosorbent Assay)
• Preparation and Storage: 2 - 8 degree C

Standard Curve (Sample)

Related Product Information for PRA elisa kit

Principle of the Assay: Prior to testing plasma samples with the PRA ELISA, a specimen pretreatment step is required. First, a protease inhibitor (PMSF) is added to the sample to prevent the degradation of angiotensin-I. Next, the generation buffer is added to bring the pH of the sample to approximately 6.0. The plasma sample is then pipetted into two aliquots. One aliquot is incubated at 0 degree C (ice bath) and the other is incubated at 37 degree C. Angiotensin-I will be generated by plasma renin in the fraction incubated at 37 degree C. The PRA ELISA is a competitive immunoassay. In the first incubation step, competition occurs between angiotensin-I present in calibrators, controls, specimen samples and an angiotensin-I-biotin conjugate (biotin conjugate) for a limited number of anti-angiotensin-I antibody binding sites on the microplate wells. During this incubation, protease inhibitors are present to prevent the degradation of angiotensin-I into smaller peptides. In the second incubation step, streptavidin-HRP conjugate is added, which binds specifically to any bound biotin conjugate. Unbound streptavidin HRP conjugate is removed by a washing step. Next, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue coloured product that is inversely proportional to the amount of angiotensin-I present. The enzymatic reaction is terminated by the addition of the stopping solution, converting the blue colour to a yellow colour. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the concentration of angiotensin-I in specimen samples and controls can be directly read. The plasma renin activity concentration in the plasma sample is calculated from the angiotensin-I concentration in the 0 degree C and 37 degree C aliquots and the generation time used. The plasma renin activity results are expressed in terms of the mass of angiotensin-I generated per volume of human plasma per unit of time (ng/mL/h).

Intended Uses: For the quantitative measurement Plasma Renin Activity (PRA) in human EDTA plasma by an ELISA (Enzyme-Linked Immunosorbent Assay).

Product Categories/Family for PRA elisa kit

Cardiovascular; Research Assays; Assay Categories