Western Blot (WB)
(NRAS Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NRAS antibody detected the NRAS protein (arrow).)
Rabbit anti-Human NRAS Polyclonal Antibody | anti-NRAS antibody
NRAS, CT (Neuroblastoma RAS Viral (v-ras) Oncogene Homolog, N-Ras, ALPS4, GTPase NRas, NS6, HRAS1, NRAS1, OTTHUMP00000013879, OTTMUSP00000023521, Transforming Protein N-Ras, v-Ras Neuroblastoma RAS Viral Oncogene Homolog) (HRP)
Gene Names
NRAS; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
NRAS; Polyclonal Antibody; CT (Neuroblastoma RAS Viral (v-ras) Oncogene Homolog; N-Ras; ALPS4; GTPase NRas; NS6; HRAS1; NRAS1; OTTHUMP00000013879; OTTMUSP00000023521; Transforming Protein N-Ras; v-Ras Neuroblastoma RAS Viral Oncogene Homolog) (HRP); anti-NRAS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human NRAS.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-NRAS antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-1:50
ELISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human NRAS (KLH).
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Western Blot (WB)
(NRAS Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NRAS antibody detected the NRAS protein (arrow).)
Western Blot (WB)
(NRAS Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NRAS antibody detected the NRAS protein (arrow).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with NRAS antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with NRAS antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(NRAS Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(NRAS Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-NRAS antibody
References
1. Smalley, K.S., Cancer Res. 68 (14), 5743-5752 (2008). 2. Banerji, U., Mol. Cancer Ther. 7(4), 737-739 (2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 21
Observed: 20
Observed: 20
NCBI Official Full Name
GTPase NRas
NCBI Official Synonym Full Names
neuroblastoma RAS viral (v-ras) oncogene homolog
NCBI Official Symbol
NRAS
NCBI Official Synonym Symbols
NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
NCBI Protein Information
GTPase NRas; N-ras protein part 4; transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog
UniProt Protein Name
GTPase NRas
Protein Family
UniProt Gene Name
NRAS
UniProt Synonym Gene Names
HRAS1
UniProt Entry Name
RASN_HUMAN
NCBI Description
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]
Research Articles on NRAS
Similar Products
Product Notes
The NRAS nras (Catalog #AAA6490544) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NRAS, CT (Neuroblastoma RAS Viral (v-ras) Oncogene Homolog, N-Ras, ALPS4, GTPase NRas, NS6, HRAS1, NRAS1, OTTHUMP00000013879, OTTMUSP00000023521, Transforming Protein N-Ras, v-Ras Neuroblastoma RAS Viral Oncogene Homolog) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NRAS can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-1:50 ELISA: 1:1,000 WB: 1:50-1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NRAS nras for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NRAS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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