Goat anti-Human NRAS Polyclonal Antibody

NRAS (Neuroblastoma RAS Viral (v-ras) Oncogene Homolog, N-Ras, ALPS4, AV095280, GTPase NRas, HRAS1, N-Ras Protein Part 4, NRAS1, OTTHUMP00000013879, OTTMUSP00000023521, Transforming Protein N-Ras, v-Ras Neuroblastoma RAS Viral Oncogene Homolog)

Cat. Num. AAA619907

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: NRAS; Polyclonal Antibody; NRAS (Neuroblastoma RAS Viral (v-ras) Oncogene Homolog; N-Ras; ALPS4; AV095280; GTPase NRas; HRAS1; N-Ras Protein Part 4; NRAS1; OTTHUMP00000013879; OTTMUSP00000023521; Transforming Protein N-Ras; v-Ras Neuroblastoma RAS Viral Oncogene Homolog); Anti -NRAS (Neuroblastoma RAS Viral (v-ras) Oncogene Homolog; anti-NRAS antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NRAS at ~21kD. Species sequence homology: mouse, rat, canine.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-NRAS antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Immunohistochemistry and Western Blot.; Dilution: ELISA: 1:32,000; Western Blot: 0.01-0.03ug/ml; Immunohistochemistry (Paraffin): 2-3ug/ml Requires HEIR using citrate buffer pH6.0
• Immunogen: Synthetic peptide, C-RMKKLNSSDDGTQ, from the internal region of human NRAS.
• Positive Control: Human lysates of cell line A431
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 monthsat -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(HEK293 overexpressing NRAS (RC202681) and probed with MBS619907. Lane 1: mock transfection. Lane 2: Human lysates of cell line A431 (calculated MW of 21.2kD according to NP_002515.1). In transfected HEK293 transiently expressing NRAS bands of ~21kD and 25kD are observed. These bands are not observed in the non-transfected HEK293)

Immunohistochemistry (IHC)

(Immunohistochemistry staining of paraffin embedded Human Small Intestnie using MBS619907 (2.5ug/ml). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

Related Product Information for anti-NRAS antibody

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML).

Product Categories/Family for anti-NRAS antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 297189567
• NCBI Official Full Name: Nras*
• Protein Family: GTPase