Rabbit anti-Human, Mouse NR3C1 Polyclonal Antibody

NR3C1, ID (NR3C1, GRL, Glucocorticoid receptor, Nuclear receptor subfamily 3 group C member 1)

Cat. Num. AAA644677

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NR3C1; Polyclonal Antibody; ID (NR3C1; GRL; Glucocorticoid receptor; Nuclear receptor subfamily 3 group C member 1); Anti -NR3C1; anti-NR3C1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-NR3C1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunofluorescence: 1:10-50; Immunohistochemistry: 1:10-50
• Immunogen: NR3C1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-272 amino acids from the Central region of human NR3C1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse liver tissue lysates (35ug/lane) using MBS644677. This demonstrates that MBS644677 detected NR3C1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma using MBS644677 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS644677 for immunohistochemistry.)

Immunofluorescence (IF)

(Fluorescent confocal image of Hela cell stained with MBS644677. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MBS644677 (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NR3C1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.)

Related Product Information for anti-NR3C1 antibody

NR3C1 is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined.

Product Categories/Family for anti-NR3C1 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 124111206
• NCBI Official Full Name: NR3C1
• Protein Family: Glucocorticoid receptor