Rabbit anti-Human NR2F6 Polyclonal Antibody | anti-NR2F6 antibody

NR2F6, NT (NR2F6, EAR2, ERBAL2, Nuclear receptor subfamily 2 group F member 6, V-erbA-related protein 2) (FITC)

Cat. Num. AAA6326614

• Gene Names: NR2F6; EAR2; EAR-2; ERBAL2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NR2F6; Polyclonal Antibody; NT (NR2F6; EAR2; ERBAL2; Nuclear receptor subfamily 2 group F member 6; V-erbA-related protein 2) (FITC); V-erbA-related protein 2; anti-NR2F6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NR2F6 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NR2F6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human NR2F6.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NR2F6 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NR2F6 antibody detected the NR2F6 protein (arrow).)

Related Product Information for anti-NR2F6 antibody

Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and-independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).

Product Categories/Family for anti-NR2F6 antibody

Antibodies; Receptors

References

Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004). Liu, X., et al. Circ. Res. 92(9):1033-1040(2003). Zhu, X.G., et al. Mol. Cell. Biol. 20(7):2604-2618(2000). Zhang, Y., et al. J. Biol. Chem. 275(4):2763-2770(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 20070199
• NCBI GeneID: 2063
• NCBI Accession #: NP_005225.2
• NCBI GenBank Nucleotide #: NM_005234.3
• UniProt Accession #: P10588; Q5XGA0; Q6P586; Q9BUE8; B2RC68
• Molecular Weight: 42,979 Da
• NCBI Official Full Name: nuclear receptor subfamily 2 group F member 6
• NCBI Official Synonym Full Names: nuclear receptor subfamily 2, group F, member 6
• NCBI Official Symbol: NR2F6
• NCBI Official Synonym Symbols: EAR2; EAR-2; ERBAL2
• NCBI Protein Information: nuclear receptor subfamily 2 group F member 6; ERBA-related gene-2; V-erbA-related protein 2; nuclear receptor V-erbA-related; v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 2
• UniProt Protein Name: Nuclear receptor subfamily 2 group F member 6
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR2F6
• UniProt Synonym Gene Names: EAR2; ERBAL2; EAR-2
• UniProt Entry Name: NR2F6_HUMAN

Uniprot Description

EAR-2: Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type- specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC). Belongs to the nuclear hormone receptor family. NR2 subfamily.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; neuron development; gene expression; steroid hormone mediated signaling; negative regulation of transcription from RNA polymerase II promoter; signal transduction; detection of temperature stimulus involved in sensory perception of pain; entrainment of circadian clock by photoperiod