Rabbit anti-Human NR1H3 Polyclonal Antibody | anti-NR1H3 antibody

NR1H3, NT (Oxysterols Receptor LXR-alpha, Liver X Receptor alpha, Nuclear Receptor Subfamily 1 Group H Member 3, LXRA)

Cat. Num. AAA6010357

• Gene Names: NR1H3; LXRA; LXR-a; RLD-1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: NR1H3; Polyclonal Antibody; NT (Oxysterols Receptor LXR-alpha; Liver X Receptor alpha; Nuclear Receptor Subfamily 1 Group H Member 3; LXRA); Anti -NR1H3; anti-NR1H3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human NR1H3. Species sequence homology: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-NR1H3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-2ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to 15aa from human LXR-A at N-terminus.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of LXR-A in rat liver tissue lysate with LXR-A antibody at (A) 1 and (B) 2ug/ml.)

Related Product Information for anti-NR1H3 antibody

Liver X receptor alpha (LXR alpha), a NR1 Thyroid Hormone-Like Receptor, has been shown to affect the transport and catabolism of cholesterol as well as fatty acid metabolism. LXR alpha also appears to be an important insulin mediating factor in the regulation of lipogenesis. LXR alpha is known to be activated by oxysterols and to regulate a wide range of genes, including members of the ATP-binding cassette (ABC) superfamily of transporter proteins (ABCA1 and ABCG1), surface constituents of plasma lipoproteins like apolipoprotein E, and cholesterol ester transport protein. The induced expression of LXR alpha may be related to foam-cell formation in atherosclerotic lesions. As a result, LXR alpha has been suggested as a potential therapeutic target for treating atherosclerosis. LXR alpha forms a heterodimer with RXR alpha and binds the DR4-type cis-element. Recently LXR alpha has been shown to act as a cAMP-responsive nuclear modulator of renin and c-myc expression, suggesting a possible involvement of LXR alpha in the cardiovascular system and in general gene transcription.

Product Categories/Family for anti-NR1H3 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 27371228
• NCBI GeneID: 10062
• UniProt Accession #: Q13133; Q8IW13; Q96H87; A8K3J9; D3DQR1
• Molecular Weight: 50,396 Da
• NCBI Official Full Name: NR1H3 protein
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1, group H, member 3
• NCBI Official Symbol: NR1H3
• NCBI Official Synonym Symbols: LXRA; LXR-a; RLD-1
• NCBI Protein Information: oxysterols receptor LXR-alpha; liver X nuclear receptor alpha variant 1
• UniProt Protein Name: Oxysterols receptor LXR-alpha
• Protein Family: Oxysterols receptor
• UniProt Gene Name: NR1H3
• UniProt Synonym Gene Names: LXRA
• UniProt Entry Name: NR1H3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the NR1 subfamily of the nuclear receptor superfamily. The NR1 family members are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. This protein is highly expressed in visceral organs, including liver, kidney and intestine. It forms a heterodimer with retinoid X receptor (RXR), and regulates expression of target genes containing retinoid response elements. Studies in mice lacking this gene suggest that it may play an important role in the regulation of cholesterol homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

LXR-alpha: Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand- binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half- sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Belongs to the nuclear hormone receptor family. NR1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor; Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nucleoplasm; nuclear chromatin; nucleus; receptor complex

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; sterol response element binding; DNA binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; cholesterol binding; steroid hormone receptor activity

Biological Process: negative regulation of lipid transport; transcription initiation from RNA polymerase II promoter; positive regulation of fatty acid biosynthetic process; intracellular receptor-mediated signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; positive regulation of toll-like receptor 4 signaling pathway; lipid homeostasis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of pinocytosis; regulation of circadian rhythm; positive regulation of cholesterol transport; cholesterol homeostasis; negative regulation of inflammatory response; positive regulation of lipoprotein lipase activity; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; sterol homeostasis; response to progesterone stimulus; negative regulation of macrophage activation; apoptotic cell clearance