Rabbit NR1H3 Polyclonal Antibody | anti-NR1H3 antibody

NR1H3 (Nuclear Receptor LXR alpha, LXR-a, LXRA, RLD-1) (FITC)

Cat. Num. AAA6429645

• Gene Names: NR1H3; LXRA; LXR-a; RLD-1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: NR1H3; Polyclonal Antibody; NR1H3 (Nuclear Receptor LXR alpha; LXR-a; LXRA; RLD-1) (FITC); anti-NR1H3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes NR1H3. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-NR1H3 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human NR1H3.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of HT29 cell lines, using NR1H3 antibody.)

Product Categories/Family for anti-NR1H3 antibody

Antibodies; Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 23503089
• NCBI GeneID: 10062
• UniProt Accession #: Q13133; Q8IW13; Q96H87; A8K3J9; D3DQR1
• Molecular Weight: 447
• NCBI Official Full Name: Oxysterols receptor LXR-alpha
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1, group H, member 3
• NCBI Official Symbol: NR1H3
• NCBI Official Synonym Symbols: LXRA; LXR-a; RLD-1
• NCBI Protein Information: oxysterols receptor LXR-alpha; liver X nuclear receptor alpha variant 1
• UniProt Protein Name: Oxysterols receptor LXR-alpha
• Protein Family: Oxysterols receptor
• UniProt Gene Name: NR1H3
• UniProt Synonym Gene Names: LXRA
• UniProt Entry Name: NR1H3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the NR1 subfamily of the nuclear receptor superfamily. The NR1 family members are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. This protein is highly expressed in visceral organs, including liver, kidney and intestine. It forms a heterodimer with retinoid X receptor (RXR), and regulates expression of target genes containing retinoid response elements. Studies in mice lacking this gene suggest that it may play an important role in the regulation of cholesterol homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

LXR-alpha: Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand- binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half- sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Belongs to the nuclear hormone receptor family. NR1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nucleoplasm; nuclear chromatin; nucleus; receptor complex

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; sterol response element binding; DNA binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; cholesterol binding; steroid hormone receptor activity

Biological Process: negative regulation of lipid transport; positive regulation of fatty acid biosynthetic process; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; positive regulation of toll-like receptor 4 signaling pathway; lipid homeostasis; negative regulation of pinocytosis; negative regulation of transcription from RNA polymerase II promoter; regulation of circadian rhythm; positive regulation of cholesterol transport; cholesterol homeostasis; negative regulation of inflammatory response; positive regulation of lipoprotein lipase activity; steroid hormone mediated signaling; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to progesterone stimulus; sterol homeostasis; negative regulation of macrophage activation; apoptotic cell clearance