Rabbit NR1D2 Polyclonal Antibody | anti-NR1D2 antibody

NR1D2 Antibody

Cat. Num. AAA9611945

• Gene Names: NR1D2; RVR; BD73; EAR-1R; REVERBB; REVERBbeta
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: NR1D2; Polyclonal Antibody; NR1D2 Antibody; BD73; EAR 1R; EAR-1R; HZF2; Nr1d2; NR1D2_HUMAN; Nuclear receptor Rev ErbA beta variant 1; Nuclear receptor Rev ErbA beta variant 2; Nuclear receptor subfamily 1 group D member 2; Orphan nuclear hormone receptor BD73; Rev erb beta; Rev erba alpha related receptor; Rev-erb-beta; REVB; REVERBB; RVR; V-erbA-related protein 1-related; anti-NR1D2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NR1D2 Antibody detects endogenous levels of NR1D2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 579

• Applicable Applications for anti-NR1D2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human NR1D2.
• Tissue Specificity: Widely expressed. Expressed at high levels in the liver, adipose tissue, skeletal muscle and brain. Expression oscillates diurnally in the suprachiasmatic nucleus (SCN) of the hypothalamus as well as in peripheral tissues.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using NR1D2 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-NR1D2 antibody

Description: Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components ARNTL/BMAL1 and CLOCK. Also regulates genes involved in metabolic functions, including lipid metabolism and the inflammatory response. Acts as a receptor for heme which stimulates its interaction with the NCOR1/HDAC3 corepressor complex, enhancing transcriptional repression. Recognizes two classes of DNA response elements within the promoter of its target genes and can bind to DNA as either monomers or homodimers, depending on the nature of the response element. Binds as a monomer to a response element composed of the consensus half-site motif 5'-[A/G]GGTCA-3' preceded by an A/T-rich 5' sequence (RevRE), or as a homodimer to a direct repeat of the core motif spaced by two nuclegotides (RevDR-2). Acts as a potent competitive repressor of ROR alpha (RORA) function and also negatively regulates the expression of NR1D1. Regulates lipid and energy homeostasis in the skeletal muscle via repression of genes involved in lipid metabolism and myogenesis including: CD36, FABP3, FABP4, UCP3, SCD1 and MSTN. Regulates hepatic lipid metabolism via the repression of APOC3. Represses gene expression at a distance in macrophages by inhibiting the transcription of enhancer-derived RNAs (eRNAs). In addition to its activity as a repressor, can also act as a transcriptional activator. Acts as a transcriptional activator of the sterol regulatory element-binding protein 1 (SREBF1) and the inflammatory mediator interleukin-6 (IL6) in the skeletal muscle.

Post Translational Modifications: Deacetylated by HDAC1. Acetylation and deacetylation regulate its transcriptional regulatory activity. Under more reducing intracellular redox conditions, Cys-384 is in its heme-bound state, which is optimal for recruitment of the NCOR1/HDAC3 corepressor complex and repression of target genes. When subjected to oxidative stress conditions, Cys-384 undergoes oxidation to form a disulfide bridge with Cys-374, also triggering a ligand switch that results in release of bound heme and derepression of target genes.

Subunit Structure: Binds DNA as a monomer or a homodimer. Interacts with NCOA5 coactivator, leading to a strong increase of transcription of target genes. Interacts (via N-terminus) with KAT5. Interacts (via C-terminus) with HDAC1. Interacts with ZNHIT1.

Similarity: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. Belongs to the nuclear hormone receptor family. NR1 subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 224177491
• NCBI GeneID: 9975
• NCBI Accession #: NP_005117.3
• NCBI GenBank Nucleotide #: NP_005117.3
• UniProt Accession #: Q14995
• Molecular Weight: Observed: 70 kDa; Predicted: 65 kDa
• NCBI Official Full Name: nuclear receptor subfamily 1 group D member 2 isoform 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1 group D member 2
• NCBI Official Symbol: NR1D2
• NCBI Official Synonym Symbols: RVR; BD73; EAR-1R; REVERBB; REVERBbeta
• NCBI Protein Information: nuclear receptor subfamily 1 group D member 2
• UniProt Protein Name: Nuclear receptor subfamily 1 group D member 2
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR1D2
• UniProt Synonym Gene Names: RVR; EAR-1R
• UniProt Entry Name: NR1D2_HUMAN

NCBI Description

This gene encodes a member of the nuclear hormone receptor family, specifically the NR1 subfamily of receptors. The encoded protein functions as a transcriptional repressor and may play a role in circadian rhythms and carbohydrate and lipid metabolism. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009]

Uniprot Description

NR1D2: Binds to the sequences 5'-AATGTAGGTCA-3' and 5'- ATAACTAGGTCA-3'. Acts as a potent competitive repressor of ROR alpha function. Belongs to the nuclear hormone receptor family. NR1 subfamily.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 3p24.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; zinc ion binding; steroid hormone receptor activity

Biological Process: transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; regulation of lipid metabolic process; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; regulation of inflammatory response; rhythmic process; lipid homeostasis; gene expression; steroid hormone mediated signaling; negative regulation of transcription, DNA-dependent; regulation of circadian rhythm