Western Blot (WB)
(Western blot analysis of extracts of Rat lung, using APLF antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 3min.)
Rabbit anti-Rat APLF Polyclonal Antibody | anti-APLF antibody
APLF Rabbit pAb
Western Blot (WB)
(Western blot analysis of extracts of Rat lung, using APLF antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 3min.)
Western Blot (WB)
(Western blot analysis of extracts of Rat lung, using APLF antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 3min.)
NCBI and Uniprot Product Information
NCBI Description
C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks (Iles et al., 2007 [PubMed 17353262]).[supplied by OMIM, Mar 2008]
Uniprot Description
APLF: Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic- apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Interacts with LIG4, PARP1, XRCC1, XRCC4 and XRCC5. Belongs to the APLF family.
Protein type: EC 4.2.99.18; DNA repair, damage
Chromosomal Location of Human Ortholog: 2p13.3
Cellular Component: nucleoplasm; cytosol; nucleus
Molecular Function: protein binding; DNA-(apurinic or apyrimidinic site) lyase activity; metal ion binding; nucleotide binding; 3'-5' exonuclease activity; endodeoxyribonuclease activity
Biological Process: regulation of isotype switching; single strand break repair; double-strand break repair; positive regulation of DNA ligation; response to DNA damage stimulus; DNA catabolic process, endonucleolytic
Research Articles on APLF
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Product Notes
The APLF aplf (Catalog #AAA9143060) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The APLF Rabbit pAb reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's APLF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the APLF aplf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LSVPAISGGN VIQGSGKEEI CKDKSQLNTT QQGRRQLISS GSSENTSAEQ DTGEECKNTD QEESTISSKE MPQSFSAITL SNTEMNNIKT NAQRNKLPIE ELGKVSKHKI ATKRTPHKED EAMSCSENCS SAQGDSLQDE SQGSHSESSS NPSNPETLHA KATDSVLQGS EGNKVKRTSC M. It is sometimes possible for the material contained within the vial of "APLF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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